目的 观察诱导分化剂苯乙酸(Phenylacetate, PA)抑制结直肠癌细胞HCT-8增殖过程中C-myc基因表达的变化.方法 体外细胞培养,运用流式细胞技术观察苯乙酸对HCT-8细胞凋亡的影响.应用原位分子杂交方法观察应用PA后对HCT-8细胞C-myc mRNA表达的情况.结果 PA组HCT-8细胞G1期比例从32.3%增加到61.0%,S期比例从59.6%下降到29.7%.PA治疗后HCT-8细胞C-myc mRNA表达阳性表达率为(12.05±7.92)%,显著低于非治疗组中的阳性率(55.15±21.64)%(P＜0.01).通过细胞周期的检测发现,PA抑制大肠癌细胞HCT-8增殖的方式为抑制细胞周期G1期比例.结论 PA通过下调结直肠癌细胞C-myc mRNA的生成以及细胞生长G1期阻滞在肿瘤诱导分化治疗方面发挥作用.%Objective To observe the change of C-myc mRNA expression in HCT-8 cell line treated by PA.Methods Cell culture in vitro and flow-cytometry (FCM) were used to evaluate cell cycle in HCT-8 cell line treated by PA(2.0 mmol/L) for seventy-two hours. Using in situ hybridization tests the changes of C-myc mRNA expression after HCT-8 cell line was treated by PA.The positive expressionrate of C-myc in PA group (12.05±7.92) % was significantly lower than in control (55.15±21.64)%(P＜0.01). Results After PA treatment, the cells growth rate were obviously inhibited and the cells arrested in G1 phase, presenting that the proportion of the cells in G1 phase was obviously increased from 32.3% to 59.6%,and that of the cells in S phase obviously decreased from 59.6% to 29.7%.Conclusion PA,a kind of differentiation inducer, could downregulate C-myc mRNA expression and induce growth arrest,differentiation and apoptosis in colorectal carcinoma.
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