小飞蓬活性成分对乳腺癌MCF-7细胞的作用

摘要

目的 通过体外实验研究小飞蓬(Erigeron canadensis L.)活性成分对乳腺癌MCF-7细胞生长、凋亡和侵袭的影响.方法 以5-氟尿嘧啶(5-FU)作用组为阳性对照组,以不加药组为阴性对照组,小飞蓬组为实验组作用MCF-7细胞.采用CCK8试剂盒测定药物对MCF-7的生长抑制率.绘制在不同药物浓度作用下的细胞生长曲线.观察不同药物浓度作用下细胞的形态学变化.利用免疫细胞化学染色技术检测药物作用后MCF-7细胞Caspase-3、MMP-9 表达情况.结果 (1)小飞蓬活性成分可抑制MCF-7细胞的生长,IC50为0.29 mg·mL-1;(2)生长曲线显示,药物作用后的MCF-7细胞生长较对照组缓慢,形态学观察显示细胞逐渐变为长,黏附性减弱;(3)药物作用后的MCF-7细胞Caspase-3 的表达强于不加药物组(P＜0.001),MMP-9 的表达明显弱于对照组(P＜0.001).结论 小飞蓬活性物质可抑制MCF-7细胞生长、侵袭,诱导凋亡.%Objective 　To study the inhibitory effect of Erigeron canadensis L.on the growth,apotosis and invasiveness of human breast cancer cells (MCF-7) in vitro .Methods 　The effects of Erigeron canadensis L .on the growth of MCF-7 cells were detected by CCK-8 kit .The MCF-7cells treated by 5-FU were used as positive control group and the MCF-7cells without any treatment as negative control group,the MCF-7cells treated by Erigeron canadensis L .were used as experimental groups .Meanwhile the growth of MCF-7 cells after treated by Erigeron canadensis L .was assessed by cell growth curve .And the morphological changes of MCF-7 cells under different concent rations of medicine were observed .Immunocytochemical staining was used to evaluate C aspase-3 and MMP-9 expression level .Results　Erigeron canadensis L .inhibited the growth of MCF-7 cells and the value of IC50 was 0.29 mg·L -1 .After treatment,the growth of MCF-7 cells slowed,and the cells became longth,adherence decreased .Drugs after the expression of Caspase-3cells w as stronger than without drug group( P ＜0 .001) .MMP9 expression was significantly weaker than the control group ( P ＜0 .001) .Conclusion 　Erigeron canadensis L .inhibit cell growth,invasion and induction of apoptosis 。