目的：探讨 SB216763联合 ABT737对肺癌细胞增殖和凋亡的作用及其机制。方法 MTT 法检测SB216763或联合 ABT737对 A549细胞增殖的影响。流式细胞术检测 SB216763或联合 ABT737对 A549细胞凋亡的影响。Western blot 检测各组内的 cleaved caspase3和 cytochrome C 蛋白表达水平。结果 SB216763抑制了 A549细胞的增殖，且呈现剂量依赖性。ABT737增强了 SB216763对 A549细胞的抑制作用。流式细胞仪结果表明ABT737增强了 SB216762诱导的细胞凋亡。Western blot 结果表明 SB216763上调了 cleaved caspase3和 cytochrome C 蛋白的表达，与 SB216763组相比，联合用药处理的细胞中 cleaved caspase-3和 cytochrome C 的表达显著增加。结论 ABT737增强 A549细胞对 SB216763的敏感性，为肺癌的治疗提供新靶点。%Objective To explore the effect of ABT737 on SB216763 induced A549 cell apoptosis and its underlying mechanism.Methods The effect of SB216763 alone or combined with ABT737 on A549 cells proliferation was em-ployed by MTT assay.Cell apoptosis rate was examined by flow cytometry analysis.Western blot was employed to ana-lyze the protein levels of cleaved caspase-3 and cytochrome C in different groups.Results The proliferation of human lung cell can be inhibited by SB216763 in a dose-dependent manner and ABT737 enhanced the inhibitory effect of SB216763.The flow cytometry data revealed that ABT737 could cause an increase in the apoptosis rate induced by SB216763.Western blot showed SB216763 increased the expression of cleaved caspase-3 and cytochrome C in A549 cell,and when SB216763 and ABT737 were combined,the expression of cleaved caspase-3 and cytochrome C were markedly enhanced.Conclusion ABT737 enhanced the sensitivity of A549 cells to SB216763,providing a new target for lung cancer theragy.
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