外源性甲胎蛋白对甲胎蛋白表达缺失HepG2细胞系增殖与凋亡的影响

摘要

Objective:To determine the effects of exogenous AFP on the proliferation and apoptosis in AFP siRNA-HepG2 cells.Methods:AFP siRNA-HepG2 cells were cultured and stimulated by AFP with the concentrations of 100μg/ml,10μg/ml,1 μg/ml,0.1 μg/ml and 0.01μg/ml respectively.The proliferation was evaluated by MTT assay.Apoptosis was stained with Annexin V-FITC/PI Kit and determined by flow cytometry.Results:MTT assay showed that 100μg/ml of AFP markedly suppressed the proliferation of AFP siRNA-HepG2,while 10μg/ml,1μg/ml,0.1 iμg/ml and 0.01 μg/ml of AFP promoted the proliferation of cells.Flow cytometry assay suggested that 100μ g/ml of AFP induced the apoptosis of AFP siRNA-HepG2 cells,while the other concentrations of AFP had no effect on cells.Conclusion:Low concentration of AFP can promote the proliferation of AFP siRNAHepG2 cells,while high concentration of AFP suppresses the proliferation and induces the apoptosis of AFP siRNA-HepG2 cells.%目的:观察不同浓度外源性甲胎蛋白(AFP)对AFPsiRNA-HepG2细胞系(AFP表达缺失HepG2细胞系)的增殖与凋亡的影响.方法:体外培养AFPsiRNA-HepG2细胞,将细胞分为对照组,100μg/ml、10μg/ml、1μg/ml、0.1μg/ml、0.01μg/ml AFP 6组,MTT法检测细胞增殖；Annexin V-APC/7-AAD染色、流式细胞术检测细胞凋亡百分比.结果:MTr结果提示,与阴性对照组比较,100μg/ml AFP组显著抑制细胞增殖(P＜0.01),低于100μg/mlAFP组促进细胞增殖(P＜0.05)；流式细胞术结果提示,与阴性对照组比较,100μg/ml AFP组显著诱导细胞凋亡(P＜0.01),低于100 μg/ml AFP干预组对细胞凋亡无影响(P＞0.05).结论:低于100μg/ml AFP可促进细胞增殖,高于100μg/ml AFP可显著抑制细胞增殖,并诱导其发生凋亡.