目的： 了解复方红芪提取液对大鼠周围神经损伤后神经元的保护作用。 方法： 实验选用雄性SD大鼠75只，随机分成5组：空白对照组、神经生长因子(NGF)对照组，中药0 .5、1.0 和1.5 ml组。以钳夹方式造成大鼠坐骨神经损伤，每日1次按组别注射 相应药物。于术后3日和1、2、4周取L46脊髓固定，切片作HE染色，光 镜下观察脊髓前角多突起运动神经元的变化。2周、4周取标本前行损伤远近段神经传导速度 测定。结果： 中药各组及NGF组在2周后其脊髓前角运动神经元数目即明显多于对照组(P均<0. 05)。2周时，中药1.5 ml组对脊髓前角运动细胞数量以及对神经纤维传导速度都有明显的 促进作用，而在4周时中药1.5 ml组和NGF组在两项指标实验中都显示出明显的促进作用 ，呈正相关关 系。结论： 复方红芪提取液对于周围神经损伤后的再生以及防止神经元坏死与NGF具有同等 的作用，并能更早期地发挥作用，其效果优于NGF。其原因可能是复方红芪制剂为多因素 、多因子共同作用的结果。%Objective: To observe the protective effects of extractive liquid of compound radix hedysari (ELCRH,复方红芪提取液) on neuron in rats with peripheral nerve injury.Methods:Seventyfive male SD rats were randomly di vided into 5 groups.The sciatic nerves were injuried by clamp.Group A: no treatment;group B:injecting neuron growth factor(NGF) 50 U/d;group C:periton eally injecting the ELCRH 0.5 ml/d;group D:in jecting ELCRH 1.0 ml /d ;group E:injecting ELCRH 1.5 ml /d.F ive groups were studied at third day,first week,second week,fourth week separate ly for the histological change in multiprocessus motor neuron of th e anterior horn of the L4L6 meanwhile at second week and fourth week for nerve c onductive velocity (NCV).Results:After 2 weeks the number of multiprocessus motor neuron of spinal anterior horn was increased significantly in group B,C,D and E than that in group A (all P<0.05). In second week， faster nerve conductive velocity and more motor neurons were fo un d in the group E(both P>0.05);in fourth week， both B and E group showed o bvious increase in NCV and the number of the motor neurons. Conclusions:The ELCRH can avoid the necrosis of nerve cell and accelerate re generati on of peripheral nerve.Its protective effects on neuron and promotive effects on peripher al nerve regeneration is better than those of NGF， otherwise the mechanisms of ELCRH action may be the results of multifactor and multimediator cooperations.
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