Objective To investigate the security of Radix et rhizome notoginseng(三七) particles, a traditional Chinese medicine for improving blood circulation and removing blood stasis, given at different time pints for treatment of rats with cerebral hemorrhage at acute stage. Methods Two hundred and ten Sprague-Dawley (SD) rats were randomly divided into two groups: treatment groups and model group. The treatment groups were subdivided into six groups whose grouping depended on the time the rats receiving the initial treatment at different time points after modeling: 0, 1, 2, 3, 5 and 7 days respectively (each group, n= 30).The injection of autologous blood into the brain was used to reproduce the rat model of cerebral hemorrhage;10 ml/kg Radix et rhizome notoginseng particles dissolved in pure water was administered intragastrically into each treatment group at corresponding time points, while the model group received an equal amount of normal saline. On the postoperative 8 days, the mortality rate was observed in each group. Rats were decapitated, the brain was taken, and the brain tissue water content and hematoma volume were measured. Results The mortality rate in treatment group receiving the drug immediately after modeling was significantly higher than that of model group (13. 3％ vs. 3.3％, P＜0. 01). Compared with the model group, in the treatment group receiving the drug immediately after modeling, the water content was significantly higher in the brain tissue [(82.80±0. 26)％ vs. (80. 97±0. 45)％, P＜0. 01], and the cerebral hematoma volume was obviously lower [(18. 92±0. 38) μl vs. (23. 96±0. 77) μl, P＜0. 01]; in the subgroup whose treatment was given on the first day after modeling, the water content of brain tissue and cerebral hematoma volume were gradually reduced,reaching the minimum on the 3rd day [brain tissue water content (74.89±0. 36)％, cerebral hematoma volume (15. 21± 0. 70) μl, compared with treatment group receiving the drug immediately after modeling all P＜0.05], afterwards began to be increased, then decreased, and on the 7th day, the brain tissue water content and cerebral hematoma volume were similar to those on the first day [brain tissue water content (78. 56±0. 65)％ vs. (78. 93±0. 51)％; cerebral hematoma volume (17. 98±0. 69) μl vs. (18. 05±0. 53) μl,both P＞0. 05]. Conclusion Radix et rhizome notoginseng particles administered at an appropriate time can play a role in improving blood circulation and removing blood stasis, that may facilitate the reduction of cerebral edema and the volume of hematoma, therefore, the treatment possesses the protective effect on the brain tissue in cases with cerebral hemorrhage.%目的 探讨脑出血急性期大鼠不同时间窗给予活血化瘀中药三七颗粒治疗的安全性.方法 将210只SD大鼠按随机数字表法分为制模后即刻及1、2、3、5、7 d给药组和模型组,每组30只;采用自体血注射法制备脑出血大鼠模型;各给药组于相应时间点将三七颗粒溶于纯净水中以10 ml/kg灌胃,模型组给予等量纯净水;术后8 d观察各组死亡率,断头处死大鼠取脑,测定脑组织含水量及脑血肿体积.结果 制模后即刻给药组大鼠死亡率明显高于模型组(13.3%比3.3%,P＜0.01).与模型组比较,制模后即刻给药组脑组织含水量明显增多[(82.80±0.26)%比(80.97±0.45)%,P＜0.01],脑血肿体积明显减少[(18.92±0.38)μl比(23.96±0.77)μl,P＜0.01];从制模后1 d起给药组脑组织含水量、脑血肿体积均逐渐减少,制模后3 d降至最低(脑组织含水量(74.89±0.36)%,脑血肿体积(15.21±0.70)μl,与制模后即刻给药组比均P＜0.05],然后开始升高,后又下降,制模后7 d与1 d时接近[脑组织含水量(78.56±0.65)%比(78.93±0.51)%;脑血肿体积(17.98±0.69)μl比(18.05±0.53)μl,均P＞0.05].结论 三七颗粒在适当时间窗给药具有活血化瘀作用,可以减轻脑水肿,减少脑血肿体积,从而对脑出血后脑组织具有保护作用.
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