三阴性乳腺癌患者中医体质类型分析及其与TOP2A基因表达的相关性

摘要

Objective To explore the correlation between TOP2A gene expression and TCM constitutional types of patients with triple-negative breast cancer.Methods The questionnaire for constitution of Chinese medicine was used to identify the TCM constitutional types of 71 patients with triple-negative breast cancer and TOP2A gene alterations were quantified by real-time PCR. 2-ΔΔCt was used to analyze the expressions of each group. SPSS17.0 was employed to analyze the correlation between TOP2A gene amplification and TCM constitutional types.Results Totally 71 patients were divided into general type (0 case), Qi deficiency type (18 cases), Yang deficiency type (10 cases), Yin deficiency type (12 cases), Qi stagnancy type (7 cases), blood stasis type (10 cases), phlegm wet calculus type (8 cases), damp-heat type (6 cases), and special type (0 case). All of them were biased constitutional types, of which Qi deficiency constitution (25.35%), Yin deficiency constitution (16.90%), blood stasis constitution (14.08%) took the majority. TOP2A gene amplification was negative correlated with each constitutional type (P<0.05).Conclusion Biased constitutional types were the main types of TCM constitutional type of patients with triple-negative breast cancer. The main three biased constitutional types of triple-negative breast cancer were Qi deficiency constitution, Yin deficiency constitution and blood stasis constitution. TOP2A gene expression in patients with triple-negative breast cancer was normal or less expressed, which predicted low chemotherapy sensitivity and poor prognosis.%目的：探讨TOPA2基因在三阴性乳腺癌中的表达特点及其与中医体质类型的相关性。方法对符合纳入标准的71例三阴性乳腺癌患者进行体质辨识，统计各体质类型分布情况。采用实时荧光定量聚合酶链反应对有肿瘤组织残存的组织蜡块进行TOP2A基因检测。采用相对定量2-ΔΔCt法分析各个样本目的基因表达的变化。采用单因素相关法对体质类型分布情况(频数)与TOP2A基因扩增表达(表达率)进行相关性分析，采用SPSS17.0统计软件进行分析。结果71例患者可分为平和质(0例)、气虚质(18例)、阳虚质(10例)、阴虚质(12例)、气郁质(7例)、血瘀质(10例)、痰湿质(8例)、湿热质(6例)、特禀质(0例)，均为偏颇体质，气虚质所占比例最大(25.35%)，阴虚质(16.90%)和血瘀质(14.08%)次之。TOP2A 基因扩增表达与各中医体质呈负相关(P＜0.05)。结论三阴性乳腺癌人群体质主要为偏颇体质，以气虚质、阴虚质和血瘀质为主。三阴性乳腺癌常见发病体质中的TOP2A基因表达呈正常或缺失状态，提示化疗敏感性低，预后差。