育肾助孕方对雷公藤多苷致卵巢储备下降大鼠卵巢形态和性激素的影响

摘要

目的：观察育肾助孕方对雷公藤多苷致卵巢储备功能下降大鼠卵巢组织形态和性激素的影响，探讨其作用机制。方法采用雷公藤多苷药液灌胃制作卵巢储备功能下降大鼠模型。48只雌性 SD 大鼠随机分为空白组、模型组、阳性药组及育肾助孕方低、中、高剂量组，育肾助孕方各剂组分别给予低、中、高浓度育肾助孕方药液灌胃，阳性药组给予戊酸雌二醇药液灌胃，模型组和空白组给予等量生理盐水灌胃，2次/d，连续14 d。观察大鼠一般情况，取卵巢称重并计算脏器指数，HE染色观察卵巢组织形态，放射免疫法检测血清雌二醇（E2）、促卵泡刺激素（FSH）、睾酮（T）水平，酶联免疫吸附法检测抑制素 B（INHB）、抗苗勒管激素（AMH）水平。结果与空白组比较，模型组卵巢组织萎缩，各级卵泡数目减少，卵巢指数和 E2、INHB、AMH水平降低，FSH、LH水平升高（P＜0.01）；与模型组比较，育肾助孕方高剂量组卵巢组织形态明显改善，各级卵泡和黄体增加，闭锁卵泡减少；卵巢指数和E2、INHB、AMH水平升高，FSH、LH水平降低（P＜0.01），且INHB与AMH具有显著相关性（P＜0.01）。结论育肾助孕方可调节性激素水平，促进卵泡生长和分泌，抑制卵泡闭锁，从而改善卵巢储备功能。%ObjectiveTo observe the effects ofYushen Zhuyun Prescription on ovarian morphology and sex hormone of rats with decreased ovarian reservation (DOR) induced by tripterygium glycosides; To explore its mechanism of action.Methods Tripterygium glycosides solution was used to establish DOR model rats by gastric gavage. 48 female SD rats were randomly divided into blank group, model group, positive medicine group andYushen Zhuyun Prescription low-, medium- and high-dose groups.Yushen ZhuyunPrescription groups were given the low, medium, high dosagesYushen Zhuyun Prescription liquid; positive medicine group were given estradiol valerate liquid; model group and blank control group were given normal saline with the same amount, by gavage 2 times per day for 14 consecutive days. The general condition of the rats was observed; ovarian was weighed and the viscera index was calculated; ovarian morphology was observed by HE staining; the levels of serum estradiol (E2), follicle stimulating hormone (FSH) and testosterone (T) were detected by radioimmunoassay; the levels of inhibin B (INHB) and anti Mullerian hormone (AMH) were detected by ELISA.Results Compared with the blank group, ovarian tissue atrophied; the number of follicles was reduced; ovarian index and the level of E2, INHB and AMH decreased; the levels of FSH and LH increased in the model group (P<0.01). Compared with the model group, ovarian tissue morphology improved significantly; the number of follicle and corpus luteum increased; follicular atresia was reduced; ovarian index and the levels of E2, INHB, and AMH increased; the levels of FSH and LH decreased in Yushen Zhuyun Prescription high-dose group (P<0.01). INHB and AMH had significant correlation (P<0.01). ConclusionYushen Zhuyun Prescription can regulate hormone levels, promote the growth and secretion of follicle, and inhibit follicular atresia, thereby improve ovarian reserve function.