寒凝证类痛经大鼠经穴左右红外温度及艾灸作用的研究

摘要

Objective To observe the surface area temperature of dysmenorrhea rats with cold stagnation syndrome; To compare the different effects of Sanyinjiao (SP6) and Guanyuan (RN4).Methods Forty female SD rats were randomized into control group, model group, Sanyinjiao (SP6) group, and Guanyuan (RN4) group, 12 rats in each group. Whole body freezing method combined with estradiol benzoate injection was used to establish models. Sanyinjiao (SP6) group and Guanyuan (RN4) group received moxibustion at corresponding points for 3 times after modeling. Infrared thermal imaging was used to measure the skin temperature at the surface projection area of Sanyinjiao (SP6) and Xuehai (SP10) and Sanyinjiao-Xuehai lines before and after moxibustion.Results Compared with control group, the temperature gap between double sides of Sanyinjiao-Xuehai lines significantly increased in model group 5-30 min after moxibustion (P<0.05). 30 min after moxibustion, the temperature of right Sanyinjiao significantly decreased in other three groups (P<0.01). Compared with model group, the temperature gap between double sides of Sanyinjiao-Xuehai lines significantly decreased after 5-30 min in Sanyinjiao group (P<0.05), while Sanyinjiao-Xuehai lines significantly decreased after 10-30 min in Guanyuan group (P<0.05). Comparison between two moxibustion groups, the influence of Sanyinjiao group to temperature gap between double sides of Sanyinjiao-Xuehai lines was earlier than Guanyuan group (P<0.05).Conclusion Moxbustion can decrease the temperature gap between double sides of Sanyinjiao-Xuehai lines and ease the imbalance. And the influence of moxbustion SP6 to temperature gap between double sides of Sanyinjiao-Xuehai lines is earlier than moxibustion RN4.%目的：观察寒凝证类痛经大鼠左右穴区及经穴连线艾灸前后红外温度，比较灸三阴交与关元效应差异。方法阴道涂片法筛选动情间期大鼠48只，随机分为正常组、模型组、三阴交组和关元组，每组12只。全身冷冻结合苯甲酸雌二醇注射法造模，治疗组分别灸双侧三阴交和关元，3壮/穴。红外热成像仪动态监测相关穴区及经络连线左右艾灸前后红外温度。结果与正常组比较，灸后5～30 min，模型组三阴交-血海经络连线左右温差显著增加（P＜0.05）；灸后30 min，其他3组三阴交右侧穴区温度显著降低（P＜0.01）。与模型组比较，灸后5～30 min，三阴交组三阴交-血海经络连线左右温差显著降低（P＜0.05），关元组10～30 min连线左右温差显著降低（P＜0.05）；两艾灸组比较，三阴交组对连线左右温差影响早于关元组。结论艾灸可显著减小经络连线左右温差，缓解失衡状态，且灸三阴交恢复失衡作用早于灸关元。