Objective To explore the clinical features of hepatitis G virus (HGV) infection and HGV replication sites in vivo. Methods A sensitive and specific reverse transcription-nested polymerase chain reaction (RT-nested PCR) was set up to detect the plus-stranded and minus-stranded HGV RNA in sera of 129 high risk population as well as in peripheral blood mononuclear cells (PBMCs) from 59 cases of them. The clinical features of HGV infection were analyzed in the meantime. Results Among 9 patients with single HGV infected, 3 cases were diagnosed as acute hepatitis, 3 cases as chronic hepatitis and the other 3 cases as healthy carriers. The detection rate of HGV RNA in PBMCs is much lower than that of HCV RNA (35.7 % vs. 77.3 % , P＜0.05). In all 59 PBMCs and 129 serum samples no minus-stranded HGVRNA was found. Conclusion HGV infection can cause acute or chronic hepatitis with mild clinical manifestation. HGV can invade PBMCs but can not replicate in PBMCs, and the ability of HGV to invade PBMCs is lower than that of HCV.%目的 探讨庚型肝炎病毒(HGV)感染的临床特点及其在体内的亲嗜性和复制位点。方法 采用逆转录-套式聚合酶链反应(RT-nested PCR)对129例高危人群的血清及其中59例的外周血单个核细胞(PBMCs)进行HGV RNA正、负链检测、临床分析。结果 9例单纯HGV感染者中，急性肝炎3例，慢性肝炎3例，另3例为无症状携带者；HGV对PBMCs的感染率(35.7%)显著低于丙型肝炎病毒(HCV)感染(77.3%，P＜0.05)；全部标本中均未发现HGV RNA负链存在。结论 HGV感染可引起急、慢性肝炎，但临床症状轻；HGV可感染PBMCs，但较HCV感染能力弱；HGV不在PBMCs中复制。
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