目的：将新型抗癌药物淫羊藿甙（ICA）和济南假单胞菌制剂（PJA）作用于PG 细胞，从肿瘤转移抑制的多个方面和环节探讨PJA、ICA抗转移作用的机制。方法：采 用粘附实验、运动侵袭实验、逆转录PCR(RT-PCR) 、细胞免疫组化等多种方法进行了检测 。 结果：PJA、ICA可降低PG细胞对胞外基质的粘附性及侵袭、运动能力，减少PG细胞表面粘附 分子CD44V6、LN-R及胞浆内CK18的表达，同时细胞内c-myc、Tiam-1基因mRNA水平均有不 同 程度的降低，而Nm23-H1 mRNA水平有不同程度的升高，且两药有明显的协同作用。结论： PJA、ICA通过对肿瘤转移多个步骤的抑制而发挥抗转移作用。%Objective: In order to further elucidate the anti-metastatic mechanism of PJA、ICA, PG cells were treated with PJA、ICA in vitro. Methods: In this study , adhesion assay, invasion assay, migration assay, RT-PCR technique and immun ohistochemical straining were used. Results:PJA、ICA could decrease PG cells adhesive ratio to laminin substrate and decreased the ability of invasion or migration. On the other hand, PJA、ICA reduced t he expression of CD44V6、LN-R and CK18 of PG cells. At the same time, th e relative expression levels of both c-myc and Tiam-1 mRNA decreased compared wi th that of the control. On the contrary, a tendency of the enhancement in the e x pression level of Nm23-H1 mRNA was also observed. Also found that PJA an d ICA had cooperative function . Conclusion: These results suggest that PJA、ICA may play anti-metastatic roles at many aspects.
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