冬虫夏草对免疫性肝损伤小鼠模型的保护作用研究

摘要

目的:观察冬虫夏草多糖(CP)对免疫性肝损伤小鼠模型的保护作用及其作用机制.方法:序贯注射卡介苗(BCG)和脂多糖(LPS)诱导小鼠产生免疫性肝损伤模型.造模过程中,小鼠每日灌胃(ig)冬虫夏草多糖(125、250、500 mg/kg),共12天.比色法测定血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)的含量、肝匀浆中超氧化物歧化酶(SOD)活力以及丙二醛(MDA)浓度,并作肝组织病理切片观察.采用ELISA法测定肝脏中TNF-α和IL-1的含量.结果:冬虫夏草多糖(125、250、500 mg/kg) ig 给药明显降低BCG+LPS诱导的免疫性肝损伤后小鼠血清中升高的ALT和AST水平,抑制肝匀浆中上升的MDA水平和升高降低的SOD活性.显著降低肝脏中TNF-α和IL-1的含量.病理检查结果显示冬虫夏草多糖有明显的保肝作用.结论:冬虫夏草多糖对小鼠免疫性肝损伤有一定的保护作用,其机制可能与降低TNF-α、IL-1的含量、平衡细胞因子有关.%0bjsctive :To observe 1he protective action of cordyceps pofysaccharrles on in m unobgical liver in jny h m ice m odel and its m echanian .M e1hods:An in m unobgical liver h jny h micemodelwas induced by adm histration of Ijpopolysaaccharide fellow ing inaction of BCG vacche into m ice fer 12 days .ALT ,AST levels h senm ,1he activity of SOD and 1he concentration of M DA in liver hem ogenates w ere exan ined,TNF-a and ti were assayed by ELBA and tie liver paihobgy was observed .Results :The experinent showed 1hat1he cordyceps pofysaccharides(125 ,250 ,500 m g/kg ,ig)cou]d reduce tie rishg ALT ,AST levels in serum of m ice w ih inm unobgical liver in jny induced by BCG+LPS ,inhabit 1he rising M DA and enhance tie activiy of SOD ,decreaseobvbus]y TNF-a,L-7L h liver hem ogenates and 1he paihobgy cut section also shewed sjgniScantiy protective effect. Conclusion :Cordyceps po^saccharides has protective effect on inm unobgical liver injiry in m ice. Item echanian is possibfy related to decrease TNF-a,L-L in liver hem ogenates and ite equilibrium on cytokine production .