Objective: To study the effect of exopolysaocharide (EPS) from Lactobacillus casei on murine gut-associated lymphocytes proliferation and production of TNF-α, IL-17 and IFN-γ Methods: intraepithelial lymphocytes (IEL) , lamina propria lymphocytes (LPL) , Peyer's Patches lymphocytes (PPL) and mesenteric lymph nodes lymphocytes (MLNL) were isolated from small intestine of mice by using Percoll discontinuous density gradient centrifugation. WST-1 assay was used to determine the proliferation of lymphocyte. The secretion of TNF-α, IL-17, IFN-γ in cell culture supernatant was assayed by ELISA. Results; EPS could induce IEL, LPL, and PPL proliferation, and increased the proliferation obviously at 100 μg/ml, EPS could promote the proliferation MNLN at 50-200 μg/ml, which was dose-dependent. EPS demonstrated inhibitory activity to the secretion of TNF-α in IEL, LPL and PPL and there were significant differences in the control group at 50-200 μg/ml. EPS could not depress the secretion of TNF-α in MLNL, and increased the secretion of TNF-α obviously at 200 μg/ml EPS demonstrated facilitative activity to the secretion of IL-17 in IEL, LPL, PPL and MLNL EPS could not influence the secretion of IFN-γ. Conclusion; EPS can induce the proliferation of gut-associated lymph-oid tissues' lymphocytes, and regulate TNF-α, IL-17 secretion.%目的:探讨干酪乳杆菌胞外多糖(EPS)对BALB/c小鼠小肠相关淋巴细胞增殖及细胞因子TNF-α、IL-17及IFN-γ分泌的影响.方法:Percoll不连续密度梯度分离法从小鼠小肠中分离上皮内淋巴细胞(IEL)、固有层淋巴细胞(LPL)、派氏集合淋巴结淋巴细胞(PPL)和肠系膜淋巴结淋巴细胞(MLNL).实验组分别加入10、25、50、100和200 μg/ml浓度的干酪乳杆菌胞外多糖.水溶性四氮唑(WST-1)法检测淋巴细胞增殖；ELISA法测定细胞上清液中细胞因子TNF-α、IL-17及IFN-γ的含量.结果:EPS能够一定程度地促进IEL、LPL、PPL的增殖,且均在100 μg/ml浓度时达到最大值,在50～ 200 μg/ml浓度范围内促进MLNL增殖呈剂量依赖性,EPS能够使IEL、LPL和PPL上清液中TNF-α含量降低,且在50～ 200 μg/ml浓度时差异极显著(P＜0.01),但对MLNL分泌TNF-α无明显抑制作用,在200 μg/ml时有显著提高(P＜0.01)；EPS可以使IEL、LPL、PPL及MLNL上清液中IL-17含量升高；EPS对IEL、LPL、PPL及MLNL上清液中IFN-γ无明显影响.结论:EPS可以促进小鼠小肠相关淋巴细胞的增殖,并能够调控细胞因子TNF-α及IL-17的分泌水平.
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