激活素 A与NGF协同刺激鸡胚背根神经节神经突起生长

摘要

目的：探讨激活素A（Activin A）与神经生长因子（Nerve growth factor，NGF）共同刺激鸡胚背根神经节（DRG）神经突起生长作用。方法：采用8 d的鸡胚DRG原代培养法，通过Activin A与NGF联合刺激，观察DRG神经突起生长和DRG神经元存活情况。采用RT-PCR检测钙基因相关肽（ CGRP）。结果：Activin A与NGF体外联合培养3 d时DRG神经突起生长比单纯NGF组更明显，DRG神经元存活数量也明显增加，Activin A与NGF联合作用可以明显促进CGRP mRNA表达。结论：Activin A对NGF诱导的鸡胚DRG神经突起生长和维持DRG神经元存活具有增强作用，提示二者的联合应用可能为治疗神经元损伤及变性疾病的应用提供了新的数据和实验依据。%Objective:To investigate the effect of activin A and nerve growth factor ( NGF) NGF on stimulating neurite outgrowth of dorsal root ganglia(DRG)of the embryonic chicken.Methods:In this study,we observed that activin A and NGF together induced neurite outgrowth of DRG and kept survival of DRG neurons by the primary cultured DRGs from embryonic day 8 ( E8 ) chicken.calcitonin gene-related peptide(CGRP)CGRP mRNA expressions were analyzed by RT-PCR.Results: The DRG treated with activin A +NGF had obvious neurite outgrowth ,compared with only NGF group on day 3,and the number of living DRG neurons also increased.Activin A +NGF up-regulated the mRNA expressions of CGRP in DRG.Conclusion:The Data demonstrated that activin A with NGF can synergistically stimulate DRG neurite outgrowth and maintain the DRG neurons survival , suggesting that it is more effective that NGF and activin A together treat the associated disease of nerve system.