高脂饮食对小鼠内脏脂肪组织T细胞亚群的影响

摘要

Objective:To study the effects of obesity induced by high-fat diet on T lymphocyte subsets in the adipose tissue in mice.Methods:C57BL/6 male mice were randomly divided into 2 groups, the normal control group and high-fat diet group.After feeding 16 weeks, serum was separated and CHOL, TG, HDL, LDL and glucose levels were measured by automatic biochemical analyzer.The concentrations of TNF-αwere determined by ELISA kit.FACS was used to analyze the number of T cells and the percentage of subgroup in epididymal fat adipose tissue.Results:Compared with control group,body weight,weight gain,epididymal fat pad weight,perirenal fat weight,blood lipids,glucose and TNF-αwere significantly increased in high-fat diet group,but there were no difference in the thymus index and spleen index between the two groups.Compared with the control group,the mice fed a high-fat diet had increasing proportion of CD3+T cells,CD4+T cells and CD8+T cells in adipose tissue and there was a significant increase on the proportion of Th1 and Th17 sublineage in the HFD group.Conclusion:High-fat diet induced obesity can lead to the increasing proportion of CD3+T cells,CD4+T cells and CD8+T cells in epididymal fat pads and generate a progressive Th1 and Th17 bias.%目的：探讨高脂饮食诱导的营养性肥胖小鼠脂肪组织内CD4＋T淋巴细胞的变化。方法：将6周龄的C57BL／6雄性小鼠随机分成2组，即正常对照组和高脂饮食组，分别饲养16周后眼底取血分离血清检测血糖、CHOL、TG、HDL和LDL水平，ELISA检测血清中TNF-α水平；游离附睾垫脂肪组织分离细胞，流式检测CD3＋、CD4＋和CD8＋T淋巴细胞的数量及亚群的比例。结果：与对照组比较，高脂饮食组体重、增加体重、附睾垫脂肪重量、肾周脂肪重量、血脂、血糖和TNF-α水平均有显著增加，但胸腺指数和脾脏指数两组间没有显著差别。脂肪组织内CD3＋、CD4＋和CD8＋T淋巴细胞数均较对照组有所增高，其中CD8＋T淋巴细胞增多最显著。在CD4＋T淋巴细胞中，Th1和Th17细胞显著增多，而Th2细胞未见显著变化。结论：高脂饮食可诱导脂肪组织内T淋巴细胞的增多，并向Th1和Th17方向极化。