miR-126在乳腺癌中与VEGF表达关系及其抗肿瘤效果

摘要

Objective:To probe the expression of miR-126 and VEGF in breast cancer, and the anti-tumor effect of miR-126. Methods:The expression of miR-126 and VEGF in breast cancer tissues and cells were detected by qRT-PCR and Western blot;after transfection with miR-126 mimics into MDA-MB-231,expression of VEGF was detected again,MTT assay and cell scratch test were used to verify the influence of miR-126 on proliferation and migration of tumor cells. Results: The expression of miR-126 was lower in the breast cancer tissues and cells,the expression of VEGF was negative correlation with it,increasing the expression of miR-126 may decrease the expression of VEGF and inhibit the proliferation and migration of breast cancer cells. Conclusion:miR-126 can reduce the proliferation and migration of breast cancer cells by inhibiting the expression of VEGF,which play an anti-tumor effect.%目的：：探讨miR-126与VEGF在乳腺癌中的表达情况,及miR-126发挥的抗肿瘤效果。方法：以qRT-PCR检测和Western blot分析miR-126与VEGF在乳腺癌组织和细胞中的表达情况；以miR-126 mimics转染乳腺癌细胞株MDA-MB-231,检测miR-126对VEGF的表达影响,MTT法和细胞划痕实验验证miR-126对肿瘤细胞的增殖和迁移能力的影响。结果：miR-126在乳腺癌组织和细胞中呈低表达,VEGF表达与其呈负相关,上调miR-126可以降低VEGF的表达,抑制乳腺癌细胞的增殖和迁移能力。结论：miR-126可以通过下调VEGF表达来实现降低乳腺癌细胞的增殖和迁移能力,从而发挥抗肿瘤效果。