目的:探讨扶正解毒通络方对肝癌HepG2细胞侵袭作用和基质金属蛋白酶(Matrix metallo proteinases,MMP)-2、MMP-9表达水平的影响及可能的作用机制.方法:CCK8实验检测扶正解毒通络方对HepG2细胞活性影响;Transwell侵袭实验检测扶正解毒通络方对HepG2侵袭能力的影响;ELISA法检测扶正解毒通络方干预HepG2细胞后 MMP-2和MMP-9的表达水平.结果:CCK8实验结果显示,扶正解毒通络方对HepG2细胞意志呈剂量时间相关性.Transwell 侵袭实验显示,2.65 mg/ml扶正解毒通络方对HepG2细胞侵袭力抑制率为52.45% (P<0.05).ELISA检测结果显示,扶正解毒通络方干预后 HepG2 细胞上清液中MMP-2 和 MMP-9表达水平下降(P<0.05).结论:扶正解毒通络方可以抑制肝癌HepG2细胞的生长和侵袭,其机制可能是通过下调MMP-2、MMP-9在肝癌细胞HepG2的表达.%Objective:To investigate the effect and mechanism of Fuzheng Jiedu Tongluofang on the expression of MMP-2,MMP-9 and invasion of human hepatoma HepG2 cell.Methods:Hepatioma HepG2 cell was treated with Fuzheng Jiedu Tongluofang.The cell viability was measured by CCK8.The invasion of HepG2 was detected by Transwell assay.The expression of MMP-2 and MMP-9 was analyzed by ELISA.Results:In the CCK8 assay,Fuzheng Jiedu Tongluofang could inhibit the proliferation of HepG2 cells at a density and time measure.In the Transwell assay,the inhibitory rate of invasion was 52.45% when treated with Fuzheng Jiedu Tongluofang at a concentration of 2.65 mg/ml. The ELISA assay indicated that the expression of MMP-2 and MMP-9 decreased significantly after treated with Fuzheng Jiedu Tongluofang(P<0.05). Conclusion:Fuzheng Jiedu Tongluofang suppressed the invasion of HepG2 with the possible mechanism of down-regulating the expression of MMP-2 and MMP-9 in HepG2.
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