目的:观察IL-35在慢性鼻-鼻窦炎伴鼻息肉的表达与意义,完善慢性鼻-鼻窦炎伴鼻息肉的发病机制.方法:随机选取2016年5月~2017年4月在我科住院的慢性鼻-鼻窦炎伴鼻息肉患者临床资料及术中鼻息肉组织.主观症状采用视觉类比法(VAS)进行评分;纤维鼻咽喉镜检查结果采用Lanza-Kennedy评分;鼻窦CT采用Lund-Mackay评分,鼻息肉组织采用Real-time PCR方法检测IL-35 mRNA(EBI3 mRNA和IL-12A mRNA)的表达水平和ELISA方法检测息肉组织中IL-35及IL-17和IL-10蛋白的表达水平.结果:共收集51例慢性鼻-鼻窦炎伴鼻息肉患者临床资料及术中鼻息肉组织,其中男性30例,女性21例,平均年龄(43.3±15.1)岁,症状评分(5.5±1.7),鼻内镜评分(6.4±2.0),CT 评分(11.7±3.8).较正常鼻黏膜组织相比,鼻息肉组织中IL-35 mRNA和蛋白均呈低表达水平,差异有统计学意义(P<0.05),而IL-17和IL-10蛋白呈高表达水平,差异均有统计学意义(P<0.05).结论:IL-35可能在慢性鼻-鼻窦炎伴鼻息肉的病理机制中起着关键的作用.%Objective:To observe the expression of IL-35 in chronic sinusitis with nasal polyps(CRSwNP),and to complement the pathogenesis.Methods:The clinical data and nasal polyp tissues of CRSwNP who were hospitalized from May 2016 to April 2017 were randomly selected.The subjective symptoms were scored by visual analogue scale (VAS),the nasal endoscopy score by Lanza-Kennedy,and the CT score by Lund-Mackay.The IL-35 mRNA were detected by Real-time PCR in nasal polyp tissues,while the IL-35, IL-17 and IL-10 were detected by ELISA.Results: A total of 51 patients of the clinical data and nasal polyp tissues were collected.Among them,30 males and 21 females had the mean age of (43.3 ± 15.1) years,the VAS score was (5.5 ± 1.7),the Endoscopy score was(6.4±2.0),the CT score was(11.7±3.8).Our results of Real-time PCR and ELISA analysis showed that IL-35 mRNA and protein expression was significantly decreased in CRSwNP compared with normal nasal mucosa (P<0.05).But IL-17 and IL-10 were significantly increased by ELISA in CRSwNP compared with normal nasal mucosa (P<0.05).Conclusion: These data suggest that IL-35 may play an important role in the development and progression of CRSwNP.
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