血浆雌激素及雌激素受体α与男性不育患者精液质量的关系

摘要

目的:探讨不同精液质量的男性患者血浆中雌激素及雌激素受体d浓度与精子浓度,活动力和形态的关系.方法:对634名男性进行精液检查,按照精液的质量参数将受试对象分成5组:正常精子组(n=147),少精子症组(n=96),弱精子症组(n=159),畸形精子症组(n=192)和非梗阻性无精子症组(n=40).采用ELISA测定各组血浆雌二醇(E2),雌激素受体d(ESRα)的浓度,比较组间差异并进行相关性分析.结果:精液正常组的血浆E2和ESRα浓度显著低于其他4组(P＜0.05),其他4组间差异无统计学为意义,血浆E2,ESRα与精子浓度,前向运动精子百分比及正常精子形态百分比呈负相关;精子浓度,血浆E2和ESRα浓度在3个年龄组间差异具有统计学意义(P＜0.05);年龄与血浆E2(r=0.114,P=0.006)和ESRα浓度(r=0.190,P＜0.001)存在正相关关系.结论:血浆E,ESRα的浓度能够反映精子的质量,在男性不育的病因分析中具有一定的指导价值.%Objective:To estimate the relationship between estradiol or estrogen receptor α in serum and semen quality of male infertile patients.Methods:Atotal of 634 men were divided to 5 groups after semen examination according to the semen quality:normozoospermiagroup (n =147),oligozoospermia group (n =96),asthenozoospermiagroup(n =159),teratospermia group(n =192),and non-obstructive azoospermia group (n =40).Levels ofestradiol(E2) and estrogen receptor α(ESRα) were detected byenzyme linked immunosorbent assay.The innergroup variances were compared,meanwhile the correlations were analyzed.Results:The level of E2 and ESRαin serumof normozoospermia group was significantly lower than thoseof oligozoospermia,asthenozoospermia,teratospermia and non-obstructive azoospermia group(P ＜ 0.05),while differences among the other 4 groups were not significant.The levels of E2 and ESRαin serum were negatively correlated with sperm concentration,forward motion or normal sperm morphology rate;The concentration of sperm,E2 and ESRα among the three age groupswere significantly different(P ＜0.05).Male age was positive correlated with the serumlevels of E2(r =0.114,P =0.006) and ESRα(r =0.190,P ＜ 0.001).Conclusions:The E2 and ESRα level in serum can reflect semen quality,which mayprovide reference and potential value in the diagnosis of male infertility.