11株经输血传播病毒（TTV）基因型分析

摘要

目的对11株经输血传播病毒（transfusion transmit virus，TTV）进行基因型分析。方法用巢式PCR扩增不同地区不明病因的急、慢性肝炎，肝硬化、肝癌和自然健康人群TTV DNA片段，并对此巢式PCR产物进行克隆，测序分析和同源性比较。结果 11例标本的222bp（引物序列除外）的TTV DNA同源性比较结果为：武汉WH1、WH2、WH3 3个患者血清中TTV DNA序列与N22同源性分别为97.0%、97.0%和98.0%；广州GZ1、GZ2、GZ3 3患者血清中TTV DNA 序列与N22同源性分别为98.0%、95.0%和95.0%；山东SD2、SD3 TTV DNA序列与N22同源性分别为94.6%和95.5%；广州GZ4、山东SD1，新疆XJ1患者TTV DNA序列与TX011同源性分别为98.0%、98.0%和95.0%。结论根据 Okamoto的分类方法，这11株TTV可属于两个亚型，即基因1型的a和b亚型。%Objective To analyze transfusion transmitted virus (TTV) genotype in hepatitis patients and healthy people. Methods DNA fragment of TTV was amplified by polymerase chain reaction with nested primers in eight patients with liver disease and three healthy persons. The nested PCR products were cloned and sequenced. Results A TTV DNA sequence of 222 bp (primer sequence excluded) was compared among the 11 subjects. The similarity between N22 and WH1, WH2, WH3, GZ1, GZ2, GZ3, SD2, SD3 was 97.0%, 97.0%, 98.0%, 98.0%, 95.0%, 95.0%, 94.6% and 95.5%, respectively. The similarity between TXO11 and GZ4, SD1, XJ1 was 98.0%, 98.0% and 95.0%, respectively. Conclusions According to Okamoto's method, the eleven TTV clones are classified into two subtypes: genotype 1a and 1b.