Objective To investigate "the effect of protease activated receptor-2 (PAR-2) on pancreatic pathological damage in severe acute pancreatitis (SAP) in rats. Methods Experimental necrotic acute pancreatitis model was established by injection of 3.5% sodium taurocholate via the pan-creatic duct. A total of 36 rats were randomized into the control group, SAP group and pancreastitis with PAR-2 activating peptide group (PAR-2 group). All the rats were sacrificed 3 hours after injec-tion of sodium taurocholate. Immunohistoehemical analysis was used to test the expression of PAR-2 in pancreas before and after the induction of SAP. Histological score of pancreatic tissue specimen was assessed microscopically. Meanwhile, the ratio of pancreatic wet dry weight, the contents of my-eloperoxidase (MPO) and Evans blue leakage rate were determined. Interleukin-6 (IL-6) in serum was tested using ELISIA. Results PAR-2 was weakly expressed in cytoplasm and membrane before SAP was induced, and the expression increased after inducing of SAP. Activating PAR-2 significantly re-duced pancreatic histological score, MPO content, wet dry weight ratio and EB leakage rate. There was correlation between pancreatic histological score and IL-6. Conclusion The expression of PAR-2 increases after inducing of SAP. PAR-2 can reduce the severity of pancreatic inflammation and plays a protective role in the local pancreatic injury.%目的 探讨蛋白酶激活受体-2(PAR-2)在大鼠重症急性胰腺炎病理损害中发挥的作用.方法 由大鼠胰胆管逆行注射3.5%牛磺胆酸钠,制作坏死性胰腺炎模型.36只大鼠被随机分为对照组、胰腺炎组、胰腺炎+皮下活化肽组(给药组),造模后3 h取材.免疫组化方法测定造模前后胰腺组织PAR-2表达情况变化;胰腺损害程度采用病理评分评价;测定胰腺组织湿/干重比、髓过氧化物酶含量,通过股静脉注射Evans blue(EB)测定EB漏出率;ELISIA方法测定血清IL-6水平.结果 正常胰腺组织胞浆及胞膜弱表达PAR-2,造模后表达升高.给药组病理评分、湿/干重比、髓过氧化物酶含量、Evans blue漏出率、血清IL-6水平明显低于胰腺炎组,胰腺病理评分与IL-6之间存在相关关系.结论 诱导重症急性胰腺炎后胰腺PAR-2表达升高,PAR-2可明显减轻胰腺炎症程度,对胰腺局部损害起保护作用.
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