低温机械灌注对大鼠供肝代谢作用的影响

摘要

目的 探索缺血30 min后肝细胞代谢变化及低温机械灌注对肝细胞代谢的影响.方法 将40只SD雄性大鼠随机均分为A、B、C、D四组.A组供肝热缺血0 min+冷保存5h;B组热缺血0 min+冷保存4h+机械灌注1h;C组供肝热缺血30 min+冷保存5h;D组供肝热缺血30 min+冷保存4h+机械灌注1h.在机械灌注过程中每隔10 min记录阻力指数值.完成灌注后切取0.2g肝组织检测肝糖原含量及丙二醛(MDA)值.结果 经机械灌注处理后肝糖原含量较冷保存有所降低,但差异无统计学意义(P＞0.05);A、B两组MDA含量无差异(P＞0.05);D组MDA含量明显高于C组(P＜0.05);经机械灌注处理后,肝脏阻力指数值均有明显降低.结论 肝脏遭受30 min热缺血损伤后,再行机械灌注,可降低其阻力指数,增加肝细胞内细胞代谢,使得脂质过氧化物生成增多.%Objective To examine the metabolic variations in liver after 30 min warm ischemic injury,and the effect of hypothermic machine perfusion on the metabolism in rats.Methods 40 SD male rats were randomly divided into 4 groups:Group A,the liver underwent warm ischemia for 0 min and cold storage (CS) for5 h; Group B,the liver was treated by warm ischemia for0 min,CS for4 h and then machine perfusion (MP) for 1 h; Group C,the liver suffered from warm ischemia for 30 min and CS for 5 h; and Group D,the liver was treated by warm ischemia for 30 min plus CS for 4 h plus MP for 1 h.During the MP process,the perfusion resistance index was recorded every 10 min,and the liver glycogen content and malondialdehyde (MDA) value were also detected.Results The hepatic glycogen content decreased after MP treatment,but there was no statistical significance (P ＞ 0.05).No differences on MDA contents was found between Group A and B (P ＞ 0.05),while MDA in Group D was significantly higher than that in Group C (P ＜ 0.05).After hypothermic MP treatment,the liver resistance index value was significantly reduced.Conclusion MP could reduce the resistance index but increase metabolic rate in liver undergoing warm ischemic injury,thus producing more lipid peroxides.