Bioflux数控剪切流活细胞检测系统在血小板和内皮功能研究中的应用

摘要

Traditional in vitro investigation of human platelets and endothelial cells is usually conducted with purified platelets and endothelial cells, but the in vitro environment is quite different from that in vivo, especially in the shear stress cells received in vivo. Most people use animal models like mouse for in vitro experiments, which is also different from that in human. To simulate the biological and physiological shear environment in vivo, we used Bioflux live cell monitoring system to test the activity of cells under different shear stress.%常规体外实验研究人血小板和内皮细胞等的功能,通常需要分离人血小板和内皮细胞,加入干预因素进行实验,但体外实验与体内环境存在较大差异,特别是细胞在体内受到的剪切力因素无法在体外重复.而体内实验通常采用小鼠等动物模型,其作用和分子机制与人类细胞往往也存在一定差异.为了更好地模拟体内环境进行细胞实验,Bioflux数控剪切流活细胞检测系统可以精确检测细胞在不同剪切力条件下的功能,更好地模拟生理和病理状态.