吉西他滨对人非小细胞肺癌A549细胞摄取18F-FDG影响的研究

摘要

目的 探讨测定人非小细胞肺癌A549细胞的18F-FDG细胞结合率方法及用吉西他滨化疗后对A549细胞摄取18FFDG的影响.方法 在不同条件下测定A549细胞的18F-FDG细胞结合率,细胞浓度5×104～1×107/瓶;18F-FDG放射性活度1.85～29.6KBq;反应时间20～120min;葡萄糖浓度0～11.1mmol/L.MTT测定加入不同剂量0～120mmol/L吉西他滨24h后细胞抑制率.测定加入不同剂量0～120mmol/L吉西他滨24h后18F-FDG细胞结合率.结果 18F-FDG细胞结合率随细胞数量、反应时间的增加而增高,随葡萄糖浓度的增高而降低,与18F-FDG放射性活度无关;加入不同剂量吉西他滨后,细胞结合率随剂量增加而下降,两者呈负相关(r=-0.78,P＜0.01).结论吉西他滨作用24h后引起人非小细胞肺癌A549细胞 18F-FDG细胞结合率下降,可用18F-FDG显像早期观测吉西他滨对人非小细胞肺癌疗效.%Objective To optimize the measurement of 18F-FDG uptake rates of non-small-cell lung cancer cell A549 and investigate the effect after administrated with Gemcitabine. Methods To detect 18F-FDG uptake rates of non-small-cell lung cancer A549 cells in different conditions:cell density ranges from 5 × 104 to 1 × 107per flask,radioactivity of 18F-FDG from 1.85 to 29.6KBq,incubating time from 20 to 120 minutes,glucose concentration from 0 to 11.1mmol/L.24 hours after administrated with Gemcitabine(0 ～ 120mmol/L),inhibition ratios and 18F-FDG uptake rates of the A549 cells were detected. Results On certain conditions,18F-FDG uptake rates of A549 cells increased as the cell number and incubating time grew,but decreased while glucose concentration raised,irrelative with radioactivity of 18F-FDG.18F-FDG uptake rates of A549 cells decreased with the concentration of Gemcitabine increasing,which presented negative correlation(r=-0.78,P＜0.01).Conclusions 18F-FDG uptake rates of non-small-cell lung cancer A549 cells decreased 24 hours after treated with Gemcitabine,which indicated early therapeutic effect of Gemcitabine for non-small -cell lung cancer could be assessed by 18F-FDG image.