雷公藤配伍金钱草抗肺癌增效作用的组成配比及其量效关系

摘要

目的：考察雷公藤（ LGT）配伍金钱草（ JQC）体外抗人非小细胞肺癌（ NSCLC） A549细胞增效作用的组成配比及其量效关系。方法将LGT与JQC按照不同质量配比（1／4、1／2、1／1、2／1、4／1）混合后，分别用水和75％乙醇回流提取，制备LGT／JQC饮片不同质量配比的提取物；采用体外MTT法检测LGT-JQC不同配比配伍提取物（200μg／ml）对人A549细胞增殖抑制的影响，确定LGT-JQC配伍增效作用的最佳组成配比；进一步采用体外MTT法检测最佳组成配比的提取物对人A549细胞增殖抑制作用的量效关系，计算其半数抑制浓度（IC50）。结果 LGT-JQC在质量配比2／1～4／1配伍的醇提物（200μg／ml）具有抑制NSCLC A549细胞增殖的增效作用（P＜0.05），且配比在2／1时增效作用最强（与配比4／1组比较， P＜0.01），而LGT-JQC配伍的水提物（200μg／ml）在既定配比下均无增效作用（P＞0.05）；LGT-JQC 配比在2／1（最佳配比）的醇提物，在浓度为50～400μg／ml范围内对A549细胞的增殖抑制呈现出良好的剂量依赖性关系，其IC50值为249.40μg／ml。结论 LGT-JQC配伍的醇提物对体外人小细胞肺癌A549细胞增殖的抑制具有协同增效作用，二者组成配比应在2／1～4／1，尤以在2／1作用为佳；LGT-JQC配比为2／1的醇提物，在浓度为50～400μg／mL对A549细胞的增殖抑制具有良好的量效关系。%Objective To investigate the component ratio and dose-effect relationship of Tripterygium wilfordii ( LGT) combined with Lysimachia Christina ( JQC) in inhibiting A549 non-small-cell lung carcinoma ( NSCLC) in vitro,for the first time.Methods Reflux extraction was used to prepare water or 75%ethanol extracts in different mass component ratios (1/4,1/2,1/1,2/1,4/1) of LGT and JQC. MTT assay was used to test the inhibition of cell proliferation and thus to determine the component ratio and dose-effect relationship of LGT combined with JQC in inhibiting NSCLC A549 cells in vitro.Besides,SPSS statistical software was used to calculate the 50%inhibitory con-centration (IC50).Results Ethanol extracts (200 μg/ml) of LGT-JQC in ratio of 2/1 to 4/1 had synergistic effect in inhibiting NSCLC A549 cell proliferation (P<0.05),and the effect of LGT-JQC in the 2/1 ratio was stronger than that of the 4/1 one (P<0.01),while the wa-ter extracts (200 μg/ml) of LGT-JQC in each given component ration had no synergistic effect (P>0.05).The ethanol extract of LGT-JQC in the 2/1 ratio at a concentration of 50~400 μg/ml range all inhibited the proliferation of A549 cells and showed a good dose-dependent manner,and its IC50 value was 249.40μg/ml.Conclusions The ethanol extract of LGT-JQC compatibility has synergistic effect on NSCLC A549 cell proliferation in vitro,the mass component ratio should be at 2/1 to 4/1,and in particular the role better in 2/1.The ethanol extract of LGT-JQC in 2/1 ratio at a concentration of 50~400 μg/ml inhibits the proliferation of A549 cells with good dose-effect relationship.