HBV患者抗-HBc单独阳性和HBV-DNA之间的相关性研究

摘要

目的：对乙型肝炎病毒（HBV）患者抗-HBc单独阳性与HBV脱氧核糖核酸（HBV-DNA）相关性进行系统分析。方法对本院2010～2013年门诊、住院部收治的5700例患者，通过荧光分析仪予以乙肝三系定量检测。将其中96例抗-HBc单独阳性患者分为两组：D组患者：63例，年龄＞50岁；X组患者：33例，年龄＜50岁。两组患者均予以HBC-DNA定量检测，并分析其结果。结果 D组患者抗-HBc单独阳性的定量检测值明显小于X组患者（P＜0.01）。X组患者中17例HBV-DNA定量检测呈阳性（51.5%），D组患者中有10例HBV-DNA定量检测呈阳性（15.9%）。两组患者HBV-DNA检测阳性率比较，差异明显（P＜0.01）。同时，D组10例患者HBV-DNA阳性定量结果范围为（1.54×103～3.25×105）copy/ml；而X组17例患者HBV-DNA阳性定量结果范围为（2.55×103～6.27×108）copy/ml，两组患者定量结果范围比较差异显著（P＜0.01）。而D组患者HBV-DNA的lg值检测与抗-HBc定量阳性之间不存在相关性（r＝0.028，P＞0.05）；但是，X组患者HBV-DNA的lg值检测与抗-HBc定量阳性之间存在相关性（r＝0.679，P＜0.05）。结论临床对HBV抗-HBc单独阳性患者，建议再结合HBV-DNA检测。同时，年龄＜50岁HBV患者单抗-HBc定量阳性与HBV-DNA的lg值检测结果呈正相关。%Objective To analyze the relationship between the the single positive HBcAb and HBV-DNA systematically. Method HBsAg, HBsAb, HBeAg and HBeAb were detected with Time Resolved Fluorescence Immunoassay in 5700 outpatients and inpatients. HBC-DNA quantitation were detected in 96 single positive HBcAb paitents. The patients were randomly divided into two groups:D group with over 50 years old and X group with under 50 years old. Result The value of HBcAb quantitation in patients over the age of 50 was signiifcantly lower than the value of HBcAb quantitation in paitents below the age of 50 (P<0.01). 17 cases were positive in HBV-DNA detection of 33 cases with single positive HBcAb below the age of 50, accounting for 51.5%. 10 cases were positive in HBV-DNA detection in 63 cases with single positive HBcAb over the age of 50, accounting for only 15.9%. There were signiifcant difference between two groups in the number of HBV-DNA positive cases (P<0.01). The lfuctuation range of 10 cases over the age of 50 with HBV-DNA positive (1.54×103~3.25×105) copy/ml, was less than that of 17 cases below the age of 50 with HBV-DNA positive (2.55×103~6.27×108) copy/ml (P<0.01). Over the age of 50, the results of HBV-DNA ( lg) and the single HBcAb quantitative positive had no statistic correlation between the two groups (r=0.028, P>0.05). Below the age of 50, the results of HBV-DNA (lg) and the single HBcAb quantitative positive had statistic correlation between the two groups (r =0.679, P < 0.05). Conclusion HBV-DNA testing is recommended in patients with single positive HBcAb quantitation. Below the age of 50, the results of HBV-DNA (lg) and the single HBcAb quantitative positive had positive correlation.