Objective: To compare the sperm parameters, sperm function and sperm acrosin activity before freezing and after thawing, and to explore the effect of sperm cryopreservation on sperm function and sperm acrosin activity. Methods: The semen samples were collected from the normal reproductive male volunteers whose semen parameters were above the lower reference limit. The semen parameters, sperm function and sperm acrosin activity before freezing and after thawing were assessed. Results: The vitality (54.96% ± 6.00% ) , the ability of spermatozoa to penetrate a column of cervical mucus (1.70 ±0.47) and the sperm acrosin activity (15.31 ±8. 87 mIU/million sperm) of the frozen - thawed sperm were significantly lower than those of the fresh samples (65. 74% ± 6. 62% , 2. 65 ± 0.49 and 54. 32 ± 36. 38 mlU/ million sperm, respectively) (P < 0.05). Conclusion: Cryopreservation of sperm can damage the sperm membrane function and the ability of spermatozoa to penetrate a column of cervical mucus in a capillary tube, and decrease the sperm acrosin activity significantly.%目的:探讨精子冷冻对精子功能和精子顶体酶活力的影响.方法:选取来自正常生育男性志愿者,且精液常规分析各参数正常的精液为标本,检测精子冷冻前后精液常规各参数、精子功能和精子顶体酶活性.结果:冷冻后精子存活率54.96±6.00％,精子宫颈黏液穿透能力1.70±0.47,精子顶体酶活性15.31±8.87mU/百万精子,与冷冻前精子存活率65.74±6.62％,精子宫颈黏液穿透能力2.65±0.49,精子顶体酶活性54.32±36.38mU/百万精子比较显著下降(P＜0.05).结论:精子冷冻导致精子膜功能和精子穿透功能受损,引起精子顶体酶活性显著下降.
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