核苷类逆转录酶抑制剂(NRTIs)对大鼠卵母细胞功能的影响

摘要

Objective:To investigate the effect of Nucleoside Reverse Transcriptase Inhibitors (NRTIs,such as AZT and 3TC) on the mice oocyte maturity and fertile potentiality.Methods and materials:After the female mice fed with AZT and 3TC,they were killed at 3,6,9 and 12 months,and the factors related to ovum maturation were detected by the method of enzyme-linked immunosorbent assay (ELISA).The changes of mice ovum maturation and the embryo developmental potentiality in-vitro culture were observed at the same time.Results:The mice ovum maturity was negatively influenced by AZT and 3TC,and the rate of embryo cleavage and blastocyst were decreased significantly after AZT and 3TC fed (P＜0.05).Conclusions:The ovum maturity and the embryo development potentiality were negatively influenced by AZT and 3TC,and the fecundity of mice decreased significantly after AZT and 3TC fed.%目的:探讨核苷类逆转录酶抑制剂(NRTIs)对大鼠卵母细胞成熟度及受精能力的影响.方法:采用NRTIs药物(AZT和3TC)灌饲雌性大鼠12个月,在用药3、6、9及12个月分别处死大鼠,以酶联免疫吸附试验法检测大鼠卵母细胞成熟相关因子的表达变化.同时观察大鼠卵母细胞体外培养成熟度及胚胎发育潜能的变化.结果:NRTIs给药后大鼠卵母细胞的成熟度受到显著影响,卵裂率和囊胚形成率均下降(P＜0.05).卵母细胞成熟相关因子MPF等的表达水平也显著下降(P＜0.05).结论:NRTIs影响大鼠卵母细胞的成熟及胚胎发育潜能,降低其生育能力.