2009-2012年一条C4a基因亚型的EV-A71优势传播链在吉林省的持续传播

摘要

Objective To analyze the transmission chains of enterovirus type71 (EV-A71) causing hand foot and mouth disease(HFMD) epidemics in Jilin province,2009-2012.Methods To choose 70 virus strains of EV-A71 to do the RT-PCR and sequences of VP1 genes,and analyzed by the software of bioedit and Mega4.0.Results All 70 EV-A71 strains belong to C4a subgenotype in Jilin province in 2009-2012,91.4％ of EV-A71 strains formed a main transmission chain.The dominant transmission chains were found in all 9 cities.Conclusion A dominant transmission chain of EV-A71 of C4a subgenotype transmitted,and caused HFMD epidemic in Jilin province.%目的 分析引起吉林省2009-2012年手足口病(hand foot and mouth disease,HFMD)流行的肠道病毒71型的传播链.方法 选择70株2009-2012年分离于吉林省HFMD病例的EV-A71分离株,对VP1编码区基因进行逆转录-聚合酶链反应扩增及RT-PCR产物的序列测定,使用Bioedit软件和MEGA 4.0软件对VP1基因编码区进行同源性、基因亲缘关系分析.结果 吉林省2009-2012年的70株EV-A71分离株均属于C4a基因亚型,91.4％的EV-A71分离株形成1个大的优势传播链,吉林省9个市、州均发现C4a基因亚型的EV-A71优势传播链.结论 2009-2012年一条C4a基因亚型的EV-A71优势传播链在吉林省持续传播,引起吉林省HFMD的暴发流行.