Objective In this study,we tested for the presence of four human coronaviruses (HCoVs) in children with respiratory tract disease in Fuzhou,Fujian,China.Methods Nasopharyngeal aspirates were collected from children with respiratory tract disease from Nov,2007 to Jan,2015.A total of 266 clinical samples were tested for HCoVs using reverse-transcription polymerase chain reaction (RT-PCR).The positive products were sequenced and compared with those in GenBank by BLAST.The positive samples were then tested for HCoV-HKU1 and HCoV-NL63 using RT-PCR method.We compared the 440 bp pol gene sequence of the 8 HCoV isolates in Fuzhou,China to other HCoV isolates documented in the GenBank database by using MEGA software version 6.06 and the neighbor-joining method.Results HCoVs were detected in 8 patients (3.0％) out of the 266 children.Two of 266 (0.38％) were positive for HCoV-HKU1;1 of 266(0.38％)were positive for HCoV-NL63;1 of 266 (0.38％) were positive for HCoV-229E;4 of 266 (1.5％) were positive for HCoV-OC43.All of children who were positive for HCoV had respiratory illness.Two HCoV-HKU1 were found to co-infect with human parainfluenza virus type 3 (HPIV-3).The 8 HCoV strains in our study fell into four clusters.Two strains of HCoV-HKU1 were genotype A.Conclusions HCoV infections were probably associated with upper and lower respiratory illness in children.Additional studies are needed to investigate the potential roles of these HCoVs in diseases.%目的 了解4种人冠状病毒(HCoV-HKU1、HCoV-NL63、HCoV-OC43、HCoV-229E)在福州急性重症下呼吸道感染患儿中的感染状况及临床意义.方法 收集2007年11月至2015年1月因呼吸道感染住进医院儿科重症监护病房的小儿鼻咽抽取物标本,共计266份.通过RT-PCR法先用一对通用引物对人冠状病毒pol基因片段进行检测.测序并BLAST比对分析8份阳性产物.对用HCoV通用引物检测阳性的标本,再分别用两种人新型冠状病毒HCoV-HKU1和HCoV-NL63的特异性引物进行扩增检测.对这8株病毒进行系统进化分析.结果 检出8例HCoV感染阳性病例,检出率3.0％.其中2例感染HCoV-HKU1,1例感染HCoV-NL63,1例感染HCoV-229E,4例感染HCoV-OC43.在这266例住院患儿中HCoV-HKU1感染的检出率约为0.75％;HCoV-NL63和HCoV-229E感染的检出率约为0.38％;HCoV-OC43感染的检出率约为1.50％.检测出两例HCoV-HKU1与人副流感病毒3型(HPIV-3)的混合感染.系统进化分析表明这8株HCoV分成4簇,2株HCoV-HKU1属于HKU1基因型A.结论 4种人冠状病毒可能是儿童下呼吸道感染中较为重要的病原.
展开▼
