Objective To observe the concentration of endothelin-1 (ET-1), nitric oxide (NO), endothelial nitric oxide synthase (eNOS) and expression of eNOS mRNA in plasma and myocardial tissue, and discuss the molecular biological mechanism of vascular endothelial system in rats with high-sodium diet. Methods SD rats (n=84) were divided randomly into high-sodium diet group (with oral 0.9% NaCL solution) and control group (with oral deionized water, each n=42). All groups were given adaptive feeding for 2 wk and experimental feeding for 8 wk. The concentration of ET-1, NO and eNOS and expression of eNOS mRNA in plasma and myocardial tissue were detected respectively. Results The level of serum sodium and plasma ET-1 concentration were significantly higher (P<0.05) and concentration of plasma eNOS and NO were significantly lower (P<0.05) in high-sodium diet group than those in control group. The concentration of NO and eNOS and eNOS mRNA expression in myocardial tissue were significantly lower (P<0.05), and ET-1 concentration in myocardial tissue was significantly higher (P<0.05) in high-sodium diet group than those in control group. Conclusion This study indicates further that high-sodium diet can induce atherosclerotic vascular lesions and target organ damage through influencing the function of vascular endothelial system.%目的 观察高钠饮食下大鼠血浆和心肌组织中内皮素-1(ET-1)、一氧化氮(NO)、内皮型一氧化氮合酶(eNOS)及eNOS mRNA等浓度,探讨高钠饮食下大鼠血管内皮系统的分子生物学机制.方法 SD大鼠84只随机分为2组: 高钠饮食组(n=42),饮用含0.9%氯化钠盐水;对照饮食组(n=42),饮去离子水.适应性喂养2周,实验喂养8周.分别检测血浆及心肌组织ET-1、NO、eNOS浓度及心肌eNOSmRNA表达.结果 高钠饮食组血钠及ET-1水平显著性高于对照组(P＜0.05),eNOS及NO显著性低于对照组(P＜0.05);高钠饮食组心肌组织NO、eNOS及eNOSmRNA显著性低于对照组(P＜0.05),心肌组织ET-1水平显著性高于对照组(P＜0.05).结论 此研究进一步说明高钠饮食通过影响血管内皮系统功能可引起动脉粥样硬化性血管病变及靶器官损害.
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