过氧亚硝酸盐对糖尿病大鼠血管收缩功能的影响的研究

摘要

Objective To observe the influence of peroxynitrite ( 0N00" ) on vasoconstrictive function of thoracic aorta in diabetic rats. Methods Male SD rats ( n = 30 ) were randomly divided into control group, diabetes group and diabetes + urate incubation ( peroxynitrite scavenger ) group ( urate group ). Taking rat thoracic aorta after removing endothelium cells as test sample, the differences in vasoconstrictive function, and expressions of RhoA/ROCK and nitrotyrosine were detected in all groups. Results The vasoconstrictive response was increased in diabetes group. The expression of RhoA mRNA was higher in diabetes group than that in urate group. The expressions of ROCK protein and nitrotyrosine increased significantly in diabetes group. Conclusion Peroxynitrite can increase the expression of ROCK protein and promote vasoconstrictive response in rats with diabetes induced by peroxynitrite.%目的 观察过氧亚硝酸盐对糖尿病大鼠胸主动脉血管收缩功能的影响.方法 雄性SD大鼠30只,大鼠随机分为3组:对照组、糖尿病组和糖尿病+尿酸盐孵育(过氧亚硝酸盐清除剂)组.以去除内皮的大鼠胸主动脉作为实验标本.分别检测各组血管收缩功能、RhoA/ROCK表达水平和硝基酪氨酸表达水平的差异.结果 糖尿病组血管的收缩反应增强.糖尿病组与尿酸盐孵育组相比,RhoA mRNA表达水平升高.糖尿病组血管ROCK的蛋白表达水平显著升高.糖尿病组血管硝基酪氨酸表达水平显著升高.结论 过氧亚硝酸盐导致糖尿病大鼠胸主动脉血管ROCK蛋白表达水平增加及血管收缩反应增强.