USBC对TNT的降解特性研究

摘要

从福安化工厂废水排放地的土壤中筛选到一株对TNT有良好降解能力的菌株,16S rDNA序列分析表明, 该菌株为Uncultured soil bacterium clone UD3(简称为USBC).研究了该菌株对TNT废水的降解特性,结果表明: 菌株USBC降解TNT的最优条件为葡萄糖1 g·L~(-1)、蛋白胨1 g·L~(-1)、细菌浓度(干重法)0.02 g·L~(-1)、pH 7、温度 30 ℃;在最优条件下反应24 h后,该菌株对100 mg·L~(-1) TNT的降解率为97.2%;菌株USBC对TNT的降解反应为一级动力学反应.%A strain isolated from a chemical plant discharge soil was used for the degradation of TNT with a high degrading efficiency. The strain was identified as uncultured soil bacterium clone UD3 (USBC) by PCR technique. Degradation of TNT in aqueous solution by the strain was studied. Results show that the optimum conditions are as follows: glucose concentration is 1 g · L~(-1); peptone concentration is 1 g · L~(-1); bacteria concentration is 0. 02 g · L~(-1); pH value is 7; temperature is 30 ℃ ; reaction time is 24 h; 97.2 % TNT is biodegraded by the strain when TNT concentration is 100 mg · L~(-1); the degradation of TNT by USBC can be described by first-order dynamic equation.