盐酸小檗碱对胰岛β细胞内质网应激相关凋亡的影响

摘要

目的 探讨盐酸小檗碱(BER)对胰岛β细胞内质网应激相关凋亡的影响. 方法 以衣霉素(Tun)诱导胰岛β细胞株MIN6细胞内质网应激,检测BER对细胞增殖、凋亡和胰岛素分泌,及对细胞凋亡相关蛋白ASK1、Caspase-12表达的影响. 结果 BER(1、10 μmol/L)对内质网应激细胞基础和葡萄糖刺激的胰岛素分泌均无明显影响,可减少细胞凋亡、抑制凋亡相关蛋白ASK1和Caspase-12的表达. 结论 BER能减少胰岛β细胞内质网应激相关凋亡,其机制可能与抑制凋亡相关蛋白ASK1和Caspase-12的表达有关.%Objective To explore the effect of berberine on the endoplasmic reticulum stress-related apoptosis in pancreatic β cells.Methods The endoplasmic reticulum stress in MIN6 cells was induced by Tunicamycin.The effect of berberine on cell proliferation and apoptosis,insulin secretion,expression of apoptosis-related proteins ASK1 and Caspase-12 were examined.Results Basal and glucose-stimulated insulin secretion were not affected while cell apoptosis was decreased by berberine (1,10 μmol/L),however,the expression of ASK1 and caspase-12 was inhibited significantly by berberine.Conclusion Berberine can reduce the endoplasrnic reticulum stress-related apoptosis in pancreatic β cells.The mechanism may be related to the inhibition of ASK1 and caspase-12 expressions.