不同中医治法对豚鼠亚急性-慢性湿疹模型疗效实验研究

摘要

目的 观察6种中医治法对豚鼠亚急性-慢性湿疹的疗效,探讨其可能的作用机制,为临床中医治疗湿疹提供可靠的理论依据.方法 采用2,4-二硝基氯苯(2,4-dinitro-1-chlorobenzene,DNCB)诱导豚鼠耳廓亚急性-慢性湿疹模型,以祛风止痒-清热燥湿解毒法(A)、清热解毒法(B)、祛风止痒法(C)、清热燥湿法(D)、清热燥湿解毒法(E)、收敛解毒法(F)6种治法的中药制剂皮肤给药2次/d,连续2周,并与生理盐水(normal saline,NS)组对照.结果 与NS组比A组减轻豚鼠耳廓肿胀最为明显,C组最低;A组脾脏系数和胸腺系数与NS组相比有明显降低(P＜0.05);各组组织切片真皮炎性细胞计数,与NS组相比均降低(P＜0.05),其中以E组最低,A组其次.结论 祛风止瘁、清热燥湿解毒法(A)能明显减轻豚鼠湿疹模型耳廓炎症肿胀、减少真皮炎细胞浸润、降低免疫器官比重系数,该治法可作为临床治疗亚急性-慢性湿疹的基本治法;其机制可能与抗迟发型变态反应、抗炎、免疫调节等作用有关.%Objective To observe the curative effects and to explore the potential mechanism of different traditional Chinese medicine treatment on subacute-chronic eczema in guinea pigs, and to provide evidence for traditional Chinese medicine treatment of eczema in clinical settings. Methods Subacute-chronic eczema models were induced by 2, 4-dinitro-chlorobenzene (DNCB), and then they were divided into six groups that were treated with the method of wind-expelling, itching-alleviating, heat-clearing, danpness-drying and detoxication(A); the method of heat-clearing anddetoxication(B); the method of expelling wind to arrest itching (C ); the method of clearing heat and drying danpness (D); the method of heat-clearing, dampness-drying and detoxication (E); and the method of wet-removing, wound healing and detoxication (F) respectively. The curative effects of traditional Chinese medicine on swelling of the outer ear, infdtration of dermal inflammatory cells, and parameters of spleen and thymus were observed and assessed. Results In group A, E and F, the discrepancies of ear thickness were significantly decreased (P<0.05) after dose administration, especially in group A. Compared to normal saline(NS)group, the discrepancies of ear weight in group A, C and D were significantly decreased (P<0.05), especally in group C. The spleen factor and thymus factor in group A was significantly decreased compared with NS group(P<0.05). The counting of hypodermic inflammatory cells in tissue slices of each group was significantly decreased compared with NS group (P<0.05). Cell counting in group E was the lowest. Conclusion The swelling of the outer ear, infiltration of dermal inflammatory cells, and parameters of spleen and thymus are significantly reduced in group A, which should be considered as the preferred treatment of subacute-chronic eczema in clinical settings. The mechanism of group A in treatment of eczema may be associated with its inhibitive role in delayed hypersensitivity and inflammation as well as its regulatory role in immune system.