PCNA蛋白在胃癌组织中的表达及其与预后的关系

摘要

目的:探讨PCNA蛋白在胃癌组织中的表达情况,并分析其与临床病理特征及预后的关系.方法:回顾性分析300例确诊并手术治疗的胃癌患者的病历资料,采用免疫组织化学SP法检测PCNA蛋白在胃癌组织及癌旁正常组织的表达,使用Kaplan-Meier法计算无复发生存率,评估PCNA蛋白与胃癌生物学特征及预后的关系.结果:癌旁正常组织中PCNA蛋白的阳性表达水平明显低于肿瘤组织(13.3%vs 36.7%,P＜0.05).PCNA蛋白的表达与胃癌患者年龄、性别、肿瘤大小以及是否有远处转移等无明显相关性(P＞0.05);而与病理组织的Lauren分型、肿瘤细胞的分化程度、是否有淋巴结转移以及TNM分期相关(P＜0.05).Kaplan-Merier曲线显示,PCNA蛋白阳性表达患者的术后无复发生存率较阴性表达患者明显降低(P＜0.001).结论:PCNA蛋白在胃癌组织中高表达,其高表达提示预后不良.%Objrctive: To investigate the expression of proliferating cell nuclear antigen(PCNA)in gastric carcinoma and its relationship with clinicopathologic factors and prognosis of gastric carcinoma. Methads: Tissue samples from 300 cases with gastric carcinoma and 15 cases with normal gastric as control group were studied. The expression of PCNA protein was detected by SP immunohistochemistry. Results: The positive rate of PCNA protein in gastric carcinoma was significantly higher than that in normal gastric tissue (13.3％ vs 36.7％,P＜0 05).The positive rate of PCNA Protein in gastric carcinoma was related to Lauren type,clinical stage, lymph node metastasis and prognosis (P＜0.05), not related to age, sex, tumor size or distant metastasis (P＞0.05). Kaplan - Merier curve shows that the survival rate of positive PCNA protein expression patients is significantly lower than that of negative PCNA protein expression patients (P＜0.001). Conclusion: PCNA protein was over expression in gastric carcinoma, and it may relate to poor prognosis.