目的:探讨结肠癌组织中Smo基因的表达及其临床意义.方法:应用免疫组织化学SP法检测96例结肠癌组织中Smo蛋白的表达,并分析其与临床病理指标及术后肝转移的关系.结果:正常结肠组织中Smo蛋白阳性表达率显著低于结肠癌组织(34.6％ vs 66.7％,P＜0.05),高T分期组、有淋巴结转移组阳性表达显著高于低T分期组及无淋巴结转移组(P＜0.05)；Smo蛋白阳性表达在性别、年龄、肿瘤大小、分化程度等组间差异无统计学意义(P＞0.05).Smo蛋白阴性表达组结肠癌术后肝转移时间显著迟于阳性表达组[(48.73±8.02)月vs (27.98±6.86)月,P＜0.01].结论:结肠癌组织中Smo蛋白异常表达参与了结肠癌的发生、发展,对结肠癌术后肝转移有一定的促进作用；Smo蛋白表达情况可以作为结肠癌术后肝转移的预测因子.%Objectives: To investigate the expression of Smo protein in colon cancer and its association with clinicopathological parameters and postoperative liver metastasis, Methods: Expression of Smo was detected by immunohistochemistry in paraffin-embedded specimens of 96 cases of colon cancer. Relationship between Smo expression and clinicopathological parameters, postoperative liver metastasis were analyzed. Results: Smo protein expression was significantly increased in colon cancer tissues compared to normal colontissues (P<0.05). Smo expression in colon tissues was increased in patients with lymph node metastases (P<0.05) and higher T stages (P<0.05). Postoperative live metastasis-free survival period was significantly longer in low Smo expression group than that of high Smo expression group (48.73 ± 8.02 vs 27.98 ± 6.86 months, P<0.01). Multi-variate analysis showed that Smo expression level is an independent prognostic factor for postoperative live metastasis-free survival. Conclusion: Colon cancer is associated with an upregulation of Smo protein expression in colon tissues. In patients with colon cancer, Smo expression level is closely related to lymph node metastases, T stages and postoperative live metastasis-free survival periods, indicative of a possible role of Smo expression in colon cancer progression.
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