目的 探讨大鼠深静脉血栓(DVT)模型股静脉内皮组织中整合素基因Itgαv(integrin alpha 5)和Itgβ3(integrin beta 3)的表达变化及其在血栓形成中的作用.方法 将60只SD大鼠随机分为对照组(10只)和模型组(50只),对模型组采用股静脉钳夹联合双下肢石膏制动构建大鼠DVT模型.不同时间点(造模后2.5 h和25 h)解剖股静脉、观测血栓的发生率,进而将模型组分为:血栓形成前组(造模后2.5 h)、血栓形成组(造模后25 h)、血栓不形成组(造模后25 h).分离股静脉内皮组织,提取总RNA;采用Genechip Rat Genome 230 2.0基因芯片筛查差异表达的基因;采用实时荧光定量聚合酶链式反应(real-time PCR) 验证这些基因的表达变化.结果 基因芯片分析及real-time PCR结果 均发现:大鼠股静脉组织中Itgαv羦和Itgβ3的表达水平在血栓形成组最高,血栓形成前组次之,均明显高于对照组和血栓不形成组(P<0.05).结论 大鼠股静脉内皮组织中Itgαv羦和Itgβ3表达水平上调可能在深静脉血栓形成中发挥了重要作用.%Objective To sludy ihe underlying role of Ilgct V (inlegrin alpha V) and Ilg(33 (inlegrin be La 3 ) in ral deep vein ihrombosis (DVT) model. Methods 60 SD rals were randomly divided inlo conlrol group ( controlled blank, n = 10) , pre - ihrombogenesis group (2. 5 hours after model buill) , ihrombogenesis group (25 hours after model buill) and non - ihrombogenesis group (25 hours after model buill). DVT ral model was established by clamping bolh femoral veins in combination wilh casl fixing. The incidence and severity degrees of ihrombus were observed by dissecling ral femoral veins in differenl lime poinls. Then lolal RNA and proleins were exlracled from ihe localized femoral venous endolhelial lissues. After gene chip -based screen, ihe gene expressions of Ilgctv and Ilg(33 were furlher idenlified by real - lime PCR ( real - lime quanlilalive polymerase chain reaction) . Results The resulls of gene chip hybridization analysis and real - lime PCR found thai the mRNA expressions of Ilgctv and Ilg(33 in ral femoral vein wall tissue were significantly up - regulated al 2. 5 hours after model buill ( pre - thrombogenesis group was higher lhan control group) (P <0. 05) , and continued up - regulating in ihrombogenesis ( thrombogenesis group was higher lhan pre - thrombogenesis group and non - ihrombogenesis group) ( P < 0. 05 ) . Conclusion The results from presenl study indicated thai increased expressions of Itgctv and Ilg(33 in local femoral venous endothelial lissue may play a crucial role in DVT.
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