神经生长因子对体外培养的人牙乳头间充质细胞增殖和细胞周期的影响

摘要

AIM: The effects of nerve growth factor on the proliferation andcell cycle of human dental papilla mesenchyme cells were detected to evaluate its possible roles in tooth development. METHODS: 3H-TdR incorporation and flow cytometer analysis. RESULTS: The nerve growth factor showed a significant concentration-dependant stimulation of [3H] thymidine uptake, with a maximum increase at 100U/ml, but inhibit the cellular proliferation at 1000U/ml. Nerve growth factor at 100U/ml increased the percenage of cells in S cycle phase and the percentage of S+G2M. CONCLUSION: nerve growth factor stimulated DNA synthesis of human dental papilla mesenchyme cells, and stimulated the transformation of cells from G1 to S cycle phase.%目的：观察神经生长因子对人牙乳头间充质细胞增殖和细胞周期的影响，探讨其在牙胚生长发育过程中的可能作用。方法：采用3H-TdR掺入和流式细胞仪分析法。结果：NGF(1～100U/ml)可明显促进培养的人牙乳头间充质细胞的3H-TdR掺入率。NGF(100U/ml)刺激细胞增殖作用最强。NGF(1000U/ml)抑制细胞的增殖。在NGF(100U/ml)作用下，细胞S%明显升高，细胞增殖指数(S+G2M)%也明显增高。结论：神经生长因子可促进人牙乳头间充质细胞DNA合成，刺激G1期细胞向S期转变。