AIM:To investigate the effects of basic fibroblast growth factor ( bFGF) on the biological char-actor of gingiva-derived mesenchymal stem cells ( GMSCs) in vitro. METHODS:GMSCs were isolated from human healthy gingival tissue samples. Proliferation, colony formation, alkaline phosphatase ( ALP) activity, STRO-1 expres-sion, osteogenic and adipogenic differentiation of GMSCs cultured in the presence or absence of bFGF, were evaluated. RESULTS:Treatment with 10 ng/mL bFGF significantly increased the proliferation and STRO-1 expression of GM-SCs. bFGF also increased oil red O staining following adipogenic induction and the expression of PPARγ and LPL of GMSCs. In contrast, it reduced the ALP activity and mineral nodule formation;inhibited the gene expression of ALP, OCN and BSP of GMSCs. CONCLUSION:Under certain conditions, bFGF enhances GMSCs stemness by up-regula-ting stem cell gene expression, increasing proliferation ability, and may regulate the differentiation potency of GMSCs.%目的：体外研究碱性成纤维生长因子( bFGF)对牙龈间充质干细胞( GMSCs)生物学特性的影响。方法：酶消化法获得原代人牙龈间充质干细胞( hGMSCs),并采用有限稀释法进行克隆纯化培养。取第3代hGMSCs,观察5~20 ng/mL不同浓度bFGF对hGMSCs增殖的影响；观察10 ng/mL bFGF对hGMSCs克隆形成能力和ALP活性的影响；通过茜素红染色、油红0染色,观察10 ng/mL bFGF对hGMSCs体外成骨、成脂分化能力的影响,并RT-PCR检测各成骨、成脂分化相关基因的表达水平；同时采用流式细胞术检测10 ng/mL bFGF对hGMSCs表面标志物STRO-1表达的影响。结果：10 ng/mL的bFGF对hGMSCs的增殖、干细胞表面标志物STRO-1的表达、hGMSCs克隆形成率、脂滴形成量以及LPL、PPARγ各成脂基因的表达水平均有明显的促进作用(P<0.05)；但对hGMSCs的ALP活性,及其成骨分化过程中矿化结节的形成量、各成骨相关基因(ALP、OCN、BSP)的表达水平均有明显的抑制作用(P<0.05)。结论：在一定的培养条件下,bFGF可增加hGMSCs的干细胞表面标志物的表达、促进hGMSCs的增殖能力；并对hGMSCs的多向分化能力起调节作用。
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