Objective To determine the optimal dosage of Folium Artemisiae argyi in air disinfection of animal facility and respiratory irritation to animals. Methods 1. Three different dosages of Folium Artemisiae argyi, 1 g/m3, 2 g/m3 and 4 g/m3, corresponding to low, middle and high dosage groups, were used in air fumigation disinfection of empty animal facility, respectively. Colony forming units (CFU) of bacteria was examined before disinfection and 12 hours after disinfection, once a week, for ten weeks. The eligible standard of CFU was ≤30, The eligible rate was calculated in these groups after 10 times of disinfection. 2. Forty SD rats were grouped into 4 groups, the control and 1 g/m3, 2 g/m , 4 g/ m dose groups, each group has a disconnector. Fumigation disinfection was done with 1 g/m3, 2 g/m and 4 g/m Folium Artemisiae argyi in different groups. The lungs of rats were taken out to observe the histopathological injury after 12 hours. Results 1. CFU assay of bacteria was not eligible in all groups before disinfection, but it was eligible in every experimental group after 10 hours disinfection. There were significant differences between the low dosage group and middle, high dosage groups, and no significant difference between middle dosage group and high dosage group. The eligible rate was asthma groups (P < 0. 05 ). In asthma group, the Eos percentage in BALF were 70% but those cells were scarcely seen in the normal group (0.08%). Eos in BALF in the experimental group were decreased markly compared with that in the asthma group (P < 0. 05 ). The histological examination of the lungs from the asthmatic animals revealed characteristic inflammatory cell infiltration in the peribronchial regions and around the submucosal blood vessels while those of experiment group displayed less inflammation and improved mucous edema and epithelial lesions of the bronchi and bronchioles. Conclusions TGS can significantly reduce airway hyperresponsiveness of the asthmatic mice.80% in low dosage group and 100% in middle and high dosage groups. 2. Pathologicl injury of lungs was observed in all experimental groups. Conclusions The optimal dosage of Folium Artemisiae argyi in air disinfection of animal facility is 2 g/m3 , meanwhile, it may irritate the respiratory system of experimental animals.%目的 研究艾叶熏蒸动物实验室空气消毒的适宜剂量及其对实验动物的吸入刺激性.方法 (1)用艾叶1 g/m3、2 g/m3、4 g/m3的低、中、高三个剂量分别对三间空动物实验室空气熏蒸消毒,检测消毒前及消毒后12 h空气菌落数(CFU),每周1次,连续10周.以CFU≤30个/皿为合格标准,计算各组消毒10次后的合格率.(2)将40只SD大鼠分为对照组和艾叶低、中、高剂量组,每组10只,放在4个隔离器内.分别用艾叶1 g/m3、2 g/m3、4 g/m3的剂量对低、中、高剂量组隔离器进行熏蒸消毒,12h后取各组大鼠肺组织,光镜下观察其损伤情况.结果 (1)消毒前,低、中、高剂量组CFU均不合格,且组间差异无显著性；消毒后12 h,各组CFU均合格,低剂量组与中、高剂量组差异显著(P＜0.05),中、高剂量组组间差异不显著；消毒10次,低剂量组合格率为80％,中、高剂量组合格率均为100％.(2)与对照组比较,艾叶三个剂量组大鼠肺组织均出现不同程度的病理变化.结论 (1)艾叶用于动物实验室空气消毒的较佳剂量为2 g/m3.(2)艾叶熏蒸空气消毒对实验动物呼吸系统有刺激性损伤.
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