猴泡沫病毒间接免疫荧光检测方法的建立及初步应用

摘要

目的 建立检测血清中猴泡沫病毒(SFV)抗体的间接免疫荧光方法,为检测实验用猴群中SFV的感染情况提供参考依据.方法 用SFV-1病毒感染BHK-21细胞,待50%细胞出现病变时,用胰蛋白酶消化细胞后以2×10<'7>/mL浓度40 μL的细胞滴到10孔镀膜的玻片上,丙酮固定.利用制备的抗原片通过间接免疫荧光法对34份猴血清标本进行检测.结果 建立了检测SFV抗体的间接免疫荧光染色方法,SFV抗体阳性19例,15例血清检测为阴性.结论 本方法具有良好的特异性,可作为SFV检测的可靠方法.%Objective Establishing the method of detecting the simian foamy virus (SFV) antibody with Immunofluorescence assay ( IFA ), to provide reference in the the detect ion of SFV infections in monkeys. Methods Selected SFV sensitive cell BHK-21, take the procedure of one step digestive treatment with trypsin while 50 percent of cell has pathological changes,titrate the cells 40 μL with the concentration of 2 × 107/mL on the slide. Then it is fixed with acetone. Testing 34 serum with the antigen slide in IFA. Results We established the immunofluorescence assay to test SFV,19 serum was positive,and 15 serum was negative. Conclusion This method can test SFV with high specificity.