BRL-37344对离体人肺脏肺泡液体清除的作用

摘要

Objective To study the effect of β3-adrenergic agonist BRL-37344 on alveolar fluid clearance (AFC) in isolated human lung and the mechanisms involved. Methods Isomolar albumin solution in the presence of 10-4 mol/L to 10-8 mol/L BRL-37344 was injected into the alveolar space in isolated human lung, and the AFC was measured. 10-5 mol/L BRL-37344 combined with phentolamine ( α-receptor antagonist), atenolol ( β1-receptor antagonist), ICI-118551 (β2-receptor antagonist), SR59230A ( β3-receptor antagonist), sodium channel blockers or Na+ /K+ -ATPase blockers were perfused into the alveolar space of isolated human lung. Results 10-4 mol/L to 10-7 mol/ L BRL-37344 significantly increased AFC in the isolated human lung, but 10-8 mol/L BRL-37344 did not increase AFC. The AFC-stimulating effect of BRL-37344 was reduced by SR59230A, amiloride and ouabain. Conclusions BRL-37344 , a β3 -adrenergic agonist, can increase AFC in isolated human lung through promoting the transport by Na +/K+ -ATP ase and accelerate the amelioration of pulmonary edema.%目的 探讨β3肾上腺素能受体激动剂BRL-37344对离体人肺脏肺泡液体清除率(AFC)的作用及其机制.方法 首先将(10-4 ～10-8)mol/L的 BRL-37344分别灌注到离体人肺脏肺泡腔内,测定AFC的变化.然后将10-5 mol/L BRL-37344分别与α受体阻滞剂酚妥拉明、β1受体阻滞剂阿替洛尔、β2受体阻滞剂ICI-118551、β3受体阻滞剂SR59230A、钠通道阻断剂氨氯吡咪、Na+-K+-ATP酶阻断剂哇巴因混合后灌注到离体人肺脏肺泡腔内,测定AFC的变化.结果 10-8 mol/L的BRL-37344对离体人肺脏肺泡液体清除率没有影响,(10-7 ～10-4)mol/L的BRL-37344能够显著提高离体人肺脏肺泡液体清除率.BRL-37344增加离体人肺脏AFC的作用能够被SR59230A、氨氯吡咪和哇巴因抑制.结论 BRL-37344主要通过调节Na+/K+-ATP酶的活性增加离体人肺脏肺泡液体清除能力,促进肺水肿液的吸收.