谷甾醇-3-O-葡萄糖苷对瘢痕疙瘩成纤维细胞增殖、凋亡及胶原合成的影响

摘要

目的 探索谷甾醇-3-O-葡萄糖苷对瘢痕疙瘩成纤维细胞(keloid fibroblasts,KF)抑制作用的研究.方法 0(对照组),3.125、6.25、12.5、25、50、100μg/mL的谷甾醇-3-O-葡萄糖苷作用KF细胞48 h后,MTT法检测细胞活力,EdU染色法检测细胞增殖,流式细胞术分别检测细胞凋亡及细胞周期,酶联免疫吸附试验(ELISA)检测细胞中COL I、COL III含量.结果 3.125、6.25、12.5、25、50、100μg/mL谷甾醇-3-O-葡萄糖苷能显著的降低KF细胞活力(P<0.01),提高细胞增殖抑制率(P<0.01),IC50=(27.54±2.18)μg/mL.与对照组比较,12.5、25、50μg/mL谷甾醇-3-O-葡萄糖苷能显著的降低细胞增殖率(P<0.01),提高细胞早期及晚期凋亡率(P<0.01),使细胞周期阻滞在G1期(P<0.01),同时还能显著的降低细胞中COL I、COL III含量(P<0.01).结论 谷甾醇-3-O-葡萄糖苷能显著的抑制KF细胞增殖,诱导细胞凋亡,同时还能抑制胶原的合成.%Objective To explore the effect of sitostero-3-O-glucoside on proliferation, apoptosis and collagen synthesis of keloid fibroblasts ( KF) . Methods Cell viability was measured by MTT assay after cells were cultured with 0 (control), 3. 125, 6. 25, 12. 5, 25, 50, 100 μg/mL sitostero-3-O-glucoside. Cell proliferation was measured by EdU staining. Cell apoptotic rate and cell cycle were measured by flow cytometry. The level of COL I and COL III was detected by enzyme linked immunosorbent assay (ELISA). Results 3. 125, 6. 25, 12. 5, 25, 50, 100 μg/mL sitostero-3-O-glucoside reduced cell viability ( P< 0. 01 ) , increased inhibitory rate of cell proliferation ( P< 0. 01 ) , IC50 =27. 54 ± 2. 18 μg/mL. Compared with the control group, 12. 5, 25, 50μg/mL sitostero-3-O-glucoside reduced the cell proliferation rate (P< 0. 01), increased the cell early and late apoptotic rates (P< 0. 01), the cell cycle was arrested at G1 phase (P< 0. 01), and the levels of COL I and COL III were down-regulated (P< 0. 01). Conclusions The results show that sitostero-3-O-glucoside can significantly inhibit the proliferation, induce cell apoptosis and inhibit the synthesis of collagen of keloid fibroblasts.