小型猪慢性心肌缺血模型的建立与无创遥测技术的应用

摘要

为建立小型猪慢性心肌缺血模型和应用无创遥测技术的评价方法,本研究采用维生素D3和异丙肾上腺素复合高脂饮食的方法建立了小型猪慢性心肌缺血模型;并利用无创遥测技术对小型猪慢性心肌缺血模型的症状进行检测与评价,同时对五指山小型猪、巴马小型猪和西藏小型猪的运输应激和高脂诱导发生动脉粥样硬化(AS)的危险因素进行评估.建立了巴马小型猪慢性心肌缺血模型与评价技术规范,明确了无创遥测技术可用于心肌缺血模型的症状检测和评价,界定了小型猪运输应激后需4周以上的适应恢复期.并发现了高脂环境下五指山小型猪、巴马小型猪和西藏小型猪发生高血脂、高血压、高胰岛素血症等AS危险因素的不同特征,这为小型猪在心血管疾病研究中的选择和应用提供参考.%In order to establish the minipig model of chronic myocardial ischemia and apply non-invasive telemetry technique, the minipig model of chronic myocardial ischemia was made induced by Vitamin D3, isoproterenol and combined with high fat diet, and the non-invasive telemetry technique was used to detect and evaluate the symptoms of minipig model of chronic myocardial ischemia.Moreover, the effects of transport stress and the risk factors of atherosclerosis (AS) induced by high fat diet among Wuzhishan minipigs, Bama minipigs and Tibetan minipigs were also evaluated.Our study has successful established the Bama minipig model of chronic myocardial ischemia and the technical specification for evaluation,.The non-invasive telemetry technique can be used to detect and evaluate the symptoms of chronic myocardial ischemia model, and defines minipigs at least need to keep for more than 4 weeks after transport stress to adaptive recovery period.In addition, the different characteristics of AS risk factors such as hyperlipidemia, hypertension and hyperinsulinemia were observed in Wuzhishan minipigs, Bama minipigs and Tibetan minipigs in high fat environment, and this provides a reference for the selection and application of minipigs in the research of cardiovascular diseases.