CYP1A1基因多态性与广西壮族原发性肝癌遗传易感性的研究

摘要

目的：探讨CYP1A1遗传多态性与广西壮族原发性肝癌遗传易感性关系。方法以60例肝细胞癌（HCC）患者（30例汉族、30例壮族）及15例非肝癌患者为研究对象，取肝癌组织和非肝癌患者肝组织标本提取DNA，用PCR-RFLP法检测CYP1A1基因多态性及基因突变分布频率。结果（1）肝癌组和对照组CYP1A1基因突变率分别为38.3%（23/60）、6.67%（1/15）（P＝0.041，OR为3.824)。（2）汉族肝癌和壮族肝癌患者 CYP1A1基因突变率分别为36.7%（11/30）、40.0%（12/30）（P＝0.791）。结论（1）CYP1A1基因突变更易发生肝癌，其危险性是CYP1A1基因未突变者发生肝癌的3.824倍。（2） CYP1A1基因型在汉族、壮族肝癌患者中分布无明显差异。%Objective To investigate the association between CYP1A1 gene polymorphism and its susceptibility to Primary Liver Cancer in the Zhuang Nationality in Guangxi. Methods To the 60 cases HCC ( 30 cases from Han nationality and 30 cases from Zhuang nationality) and 15 case from control population of non hepatocellular carcinoma, the genetype and genetic mutation frequency of CYP1A1 were determined by polymerase chain reaction combined with restriction fragment length polymorphism method(PCR-RFLP). Results (1)Among the HCC group and the control group, The genetic mutation of CYP1A1 were 38.3%(23/60) and 6.67% (1/15) respectively, the difference is significant (P=0.041, OR3.824). (2)Among the HCC of Han nationality and Zhuang nationality, The genetic mutation of CYP1A1 were 36.7%(11/30) and 40.0%(12/30) respectively, the difference was not significant(P = 0.791). Conclusions (1) The CYP1A1 genetype has high susceptibility ofhepatocarcinogenesis, the risk of CYP1A1genetic mutation is 3.824 times than non-mutation. (2) The CYP1A1 genetype is not significant difference between the HCC in Han and Zhuang nationality.