肺癌转移相关microRNA miR-29b的生物信息学分析

摘要

Objective:This paper aims to bioinformatically analyze the target genes of miR-29b and to provide clues for cancer research targeting miR-29b. Methods:The differential expression levels of miRNAs in CD133+and CD133-A549 cells were detected using the miRNA PCR chip. Real-time polymerase chain reaction was performed to verify the partially differential expression of miR-NAs. Target genes of miR-29b were predicted by miRecords and analyzed by gene ontology and signal transduction pathway enrich-ment analysis. Results: The miR-29b expression was significantly decreased in the CD133+A549 cells compared with that in the CD133-cells. The number of miR-29b target genes was 106. The functions of these target genes were enriched in binding and extracel-lular matrix structural constituent (P<0.01). The JAK-STAT and TGF-βsignal transduction pathways were significantly enriched (P<0.05). Conclusion:The abnormal expression of miR-29b may be related to metastasis. Some of the predicted target genes of miR-29b were significantly enriched in the signaling pathways in relation to the tumors.%目的：应用生物信息学分析A549细胞中在CD133阳性低表达的miR-29b的靶基因及其功能，为以miR-29b为靶点的肿瘤研究提供线索。方法：利用miRNA PCR芯片筛选A549细胞中CD133阳性和CD133阴性差异表达的miRNA，选用miRecords预测miR-29b的靶基因，合并已证实的靶基因，利用GOEAST和DAVID数据库对所得靶基因进行功能富集分析和信号转导通路富集分析。结果：A549细胞中与CD133阴性比较，miR-29b在CD133阳性中表达下调。miR-29b靶基因有106个，其靶基因功能富集于结合和细胞外基质形成等作用（P<0.01）；信号转导通路显著富集于JAK-STAT和TGF-β等信号转导通路（P<0.05）。结论：miR-29b可能与肺癌转移相关，miR-29b的靶基因显著富集在与肿瘤相关的信号通路中。