Toonaciliatin A在体内外抑制胃癌MKN45细胞增殖并诱导细胞凋亡

摘要

Objective:To investigate the effects of traditional Chinese medicine monomer Toonaciliatin A on proliferation and apoptosis of human gastric cancer cell line MKN45invitroandinvivo.Methods:MKN45 cells were cultured invitroand treated with Toonaciliatin A (15,30,and 60μg/mL)for 48 h.Then the cell viability was measured by MTT assay.The apoptosis were determined by flow cytometry using Annexin V-FITC/PI assay.The BALB/c nude mouse xenograft model of MKN45 cells was established and administrated with Toonaciliatin A (10,20,and 40 mg/kg)for 21 days,then the weights of xenograft tumors and mouse were calculated,and the expression of caspase-3 protein in tumor tissues was detected by immunohistochemical method.Results:As compared with the control group,Toonaciliatin A inhibited effectively the proliferation of MKN45 cells,and induced apoptosis of MKN45 cellsinvitro(P<0.05).In the nude mouse xenograft model of MKN45 cells,the tumor weight of Toonaciliatin A treatment group was significantly decreased as compared with that of the control group (P<0.05 ).Toonaciliatin A induced the expression of caspase-3 proteininvivo (P<0.05 ).Conclusions:Toonaciliatin A is an effective anti-tumor Chinese medicine monomer.%目的:探讨中药单体Toonaciliatin A体内外对人胃癌MKN45细胞增殖和凋亡的影响.方法:体外培养MKN45细胞,采用不同质量浓度的Toonaciliatin A(15、30、60μg/mL)分别处理48 h,MTT法检测细胞增殖活性;Annexin V-FITC/PI法染色后流式细胞仪检测细胞凋亡率.建立人胃癌MKN45细胞的BALB/c裸鼠移植瘤模型,采用不同剂量的Toonaciliatin A(10、20、40 mg/kg)给药21 d,检测移植瘤质量及裸鼠体质量;免疫组织化学法检测各组移植瘤组织中caspase-3蛋白的表达水平.结果:Toonaciliatin A体外可有效抑制MKN45细胞增殖,并诱导MKN45细胞凋亡,药物处理组与对照组相比差异有统计学意义(P<0.05).与对照组相比,Toonaciliatin A给药组移植瘤质量明显降低(P<0.05).Toonaciliatin A体内可诱导移植瘤组织中caspase-3蛋白表达增加(P<0.05).结论:Toonaciliatin A体内外能有效抑制胃癌MKN45细胞增殖并诱导细胞凋亡.