天七方对过氧化氢诱导心肌细胞损伤模型核因子 E2相关因子2／抗氧化反应元件通路的影响

摘要

Objective To study the effect of TianQi Formula (TQ)on the protection of H2 O2-induced card-iocytes apoptosis via Nrf2/ARE passway,and to explore the mechanism of protection.Methods The model of H2 O2-induced apoptosis of cardiocytes was used to determine the protective effects of TQ (MTT).The expression of Nrf2/ARE-related proteins was determined with Western blotting assay.Result Compared with normal group,TQ of the different dose alone showed no cytotoxicity and had significant protective effects against H2 O2-induced apoptosis of cardiocytes in a dose-dependent manner(P<0.01).Moreover,TQ significantly strengthened the protein expression of Nrf2 and HO-1 .Conclusion TQ can prevent H2 O2-induced apoptosis in cardiocytes,the mechanism might be related to the regulation of Nrf2/ARE passway.%目的：探讨天七方（TQ）对过氧化氢（H2 O2）所致心肌细胞损伤的核因子E2相关因子2/抗氧化反应元件（Nrf2/ARE）通路的影响，阐明TQ抗心肌损伤的分子机制。方法建立H2 O2诱导的心肌损伤模型，通过MTT法观察在不同浓度TQ的干预下，对心肌细胞增殖作用的影响，Western blot法检测Nrf2/ARE通路相关蛋白表达情况。结果与空白组相比，TQ对心肌细胞无明显损伤，并且TQ可减少H2 O2所导致心肌细胞损伤（P＜0．01）。此外，TQ可上调蛋白Nrf2和HO-1的表达。结论 TQ通过激活Nrf2/ARE通路，增加蛋白HO-1的表达，抑制H2 O2所致的心肌细胞的氧化损伤。