胃癌组织中Apollon和Caspase 9的表达及相互关系

摘要

目的 探讨凋亡相关基因Apollon和Caspase 9在胃癌组织中的表达、临床意义及相关性.方法 应用免疫组化SP法检测105例胃癌组织、38例癌旁组织及29例正常胃组织中Apollon和Caspase 9蛋白的表达,并分析Apollon和Caspase 9的表达与胃癌临床病理特征的关系.结果 Apollon在胃癌组织、癌旁组织及正常胃组织中分别有82例(78.10％)、8例(21.05％)和2例(6.90％)阳性,差异有显著性(P < 0.01); Apollon蛋白在胃癌中的表达与肿瘤分化程度、TNM分期及淋巴结转移相关(P < 0.05),与其它临床病理特征无关(P > 0.05); Caspase 9在胃癌组织、癌旁组织及正常胃组织中分别有21例(20.00％)、23例(60.53％)和21例(72.41％)阳性,差异有显著性(P < 0.01); Caspase 9蛋白在胃癌中的表达与肿瘤分化程度、TNM分期及淋巴结转移相关(P < 0.05),与其它临床病理特征无关(P > 0.05); Apollon和Caspase 9在胃癌组织中的表达呈显著负相关(r = - 0.541 1,P < 0.01).结论 Apollon和Caspase 9的相互作用可能参与胃癌的发生、发展; Apollon与胃癌的浸润、转移密切相关,可作为潜在的治疗靶点.%Purpose To explore the expression,significance and relationship of apoptosis related gene Apollon and Caspase 9 in gastric carcinoma. Methods The SP immunohistochemical method was used to detect the expression of Apollon and Caspase 9 in 105 cases of gastric carcinoma,38 adjacent tissues and 29 normal tissues,and the expression of Apollon and Caspase 9 was analyzed with relation to clinicopathologic factors. Results The numbers of positive expression of Apollon gene in gastric carcinoma tissues,adjacent tissues and normal tissues were 82(78. 10％) ,8(21. 05％) and 2(6. 90％) respectively, there was significant difference between gastric carcinoma tissues, adjacent tissues and normal tissues (P < 0. 01) . The expression of Apollon in gastric carcinoma was positively correlated with degree of tumor differentiation,TNM staging and lymph node metastasis (P < 0. 05) ,but not with other clinicopathologic factors (P > 0. 05) . The numbers of positive expression of Caspase 9 gene in gastric carcinoma tissues,adjacent tissues and normal tissues were 21 (20. 00％) ,23 (60. 53％) and 21 (72. 41％) ,respectively,and there was significant difference between gastric carcinoma tissues,adjacent tissues and normal tissues (P < 0. 01) . The expression of Caspase 9 in gastric carcinoma was positively correlated with degree of tumor differentiation, TNM staging and lymph node metastasis (P < 0. 01) ,but not with other clinicopathologic factors (P > 0. 05) . The expression of Apollon was negatively correlated to Caspase 9 in gastric carcinoma with statistical significance (r = - 0. 541 1,P < 0. 01) . Conclusions The interaction of Apollon and Caspase 9 may be involved in the gastric carcinogenesis and progression. Apollon is closely related with invasion and metastasis of gastric carcinoma,and it may be a potential treatment target.